Radislav Sedláček

This study shows TMEM55B and TMEM55A regulate lysosome distribution in neurons by affecting motor protein transport, with TMEM55B specifically promoting anterograde transport of LAMP1-positive organelles.

The paper shows that prior antigen exposure redirects CD8 T-cell activation programs, shaping progeny fate and persistence through distinct stemness and effector programs in naive and memory cells.

This study establishes a mouse model of Experimental Autoimmune Adrenalitis (EAA) and identifies IFNG-producing CD4+ T cells as key drivers of autoimmune adrenalitis.

This paper shows that asparagine enables transsulfuration to restore redox balance in tumors lacking aspartate synthesis enzymes, highlighting transsulfuration as a targetable vulnerability.

The paper identifies de novo EHMT2 variants causing an autosomal dominant Kleefstra syndrome by impairing G9a methyltransferase activity, mimicking EHMT1 haploinsufficiency.

This study identifies FAM83H as a regulator linking keratin organization to Notch signaling in epidermal morphogenesis, showing its role in keratinocyte differentiation and cell-cell adhesion.

This study shows that iRhom2 deficiency reduces acute lung inflammation in mice by impairing ADAM17-mediated shedding of inflammatory mediators and neutrophil recruitment.

The paper develops a UNet-based model for automated retinal dysplasia segmentation in mouse OCT scans, achieving high accuracy and consistency with expert annotations.

This paper describes a method to co-isolate AAV with extracellular vesicles for efficient genome editing in rodent embryos, achieving higher viability and targeting efficiency compared to traditional purification methods.

This study shows that FAM83H is essential for thymic stroma organization and T cell development, as its deficiency leads to impaired thymic architecture and reduced T cell production.

This study shows TENT5A polyadenylates enamel matrix protein mRNAs, enhancing their stability and translation, which is essential for proper enamel mineralization.

This study shows that LCK deficiency in CD8 T cells reduces proliferation but increases effector T-cell formation in mice, revealing its dual role in T-cell responses.

This study establishes TTE reference ranges for mice, showing anesthesia significantly affects cardiac function, with minimal sex differences and modest age-related changes.

The study identifies Claudin 1 on DC1s as essential for their positioning near mTECs, facilitating antigen transfer and DC1 maturation, which maintains central tolerance.

The paper analyzes 3,313 genetically engineered mouse lines to identify gene essentiality as the main factor affecting Cas9-based genome editing success.

The paper shows that enhancing RNAi in testes via oocyte-specific Dicer does not rescue spermatogenic defects caused by Mili loss.

This study identifies ABIN1 as a negative regulator of effector functions in cytotoxic T cells, showing its role in suppressing NF-κB and p38 pathways.

This study introduces mitoDFX, a mitochondrially targeted iron chelator that simultaneously depletes iron and induces ferroptosis, showing high selectivity and efficacy against cancer cells.

This study shows that Dicer{Delta}HEL1/wt mice exhibit increased endogenous siRNA levels and functional RNAi in vivo, providing a new model for studying mammalian canonical RNAi.

This study shows that mutations in Prpf8 in mice lead to misexpression of circRNAs and cerebellar granule neuron degeneration.

The paper investigates the role of the adipose tissue-specific lncRNA Gm15551 in BAT activation through transcriptional profiling and phenotyping, finding no significant effect on adipocyte function.

This study identifies the DExD/H helicase domain of Dicer as critical for accurate miRNA biogenesis, showing its role in substrate recognition, cleavage, and strand selection in mammals.

This study constructs a single-cell and spatial atlas of the choroid plexus in developing and adult mice, revealing cell types, regional gene expression, and cell-cell interactions.

This study identifies the MEIS-WNT5A axis as critical for the development and morphology of the fourth ventricle choroid plexus.

The paper reports the generation of over 5,000 new mouse mutant strains for targeted genes, significantly expanding the available genetic resources for biomedical research.

This study shows that plectin is essential for intestinal epithelial integrity and protects against colitis by maintaining mechanical stability and junctional function.

This study investigates immune and hematopoietic system alterations in Bardet-Biedl Syndrome patients and a mouse model, finding increased autoimmune disease incidence, elevated white blood cell counts, and B cell abnormalities.

The paper integrates gene essentiality data from human and mouse screens to classify genes into FUSIL categories, identifying developmental lethal genes associated with rare diseases.

This study shows CRMP2 is essential for Sema3F-dependent axon pruning and dendritic spine remodeling, linking its dysfunction to ASD-like behaviors in mice.

This study identifies TENT5A as a cytoplasmic poly(A) polymerase essential for collagen production and bone formation, revealing its role in osteogenesis imperfecta type XVIII.