Matthias P. Lütolf

This study creates a perturbation atlas of mouse neural organoids to understand macroglial reactivity and identify drivers of degeneration.

This paper establishes a human lung explant model to quantify T cell bispecifics' on-target, off-tumor toxicity by measuring T cell activation and epithelial cell killing.

This study shows that growth factor-free synthetic matrices reveal donor-specific differences in human skeletal progenitor cell osteogenic potential, which are masked by conventional methods.

This study maps human B-cell populations and their interactions across 10 tissues using single-cell RNA sequencing, revealing tissue-specific adaptations and residency gene modules.

The paper develops a human urothelial organoid model to study urine-urothelium interactions in UTI resilience and UPEC recurrence, finding that high-solute urine weakens tissue and promotes CWD UPEC formation.

The paper develops a limb organoid model to study how signaling centers direct cell fate and spatial organization during limb development.

The paper introduces a platform for high-throughput spatial phenotyping of multilineage organoids, identifying pathway modulators and treatment strategies for PDAC.

This paper introduces a scalable platform to generate regionally specified neural organoids from uniform single-lumen neuroepithelium, improving reproducibility and fidelity to natural brain development.

This study uses deep learning and spatial data to show how tumor architecture and cell states co-evolve, influencing colorectal cancer outcomes.

This study identifies mRNA deadenylation as a key mechanism regulating body plan formation in mouse gastruloids by affecting mesoderm and endoderm differentiation.

The paper uses live imaging and single-cell transcriptomics to study how extracellular matrix influences brain organoid development and regionalization.

The paper develops engineered human gut tissues to study host-microbe interactions and identifies an epithelial gene regulatory network in response to Salmonella Typhimurium infection.

This paper creates a human endoderm organoid cell atlas by integrating single-cell transcriptomes from multiple organoid types to compare cell states with primary tissues and disease models.

The paper introduces a microwell array-based thymic organoid model that supports T-cell development and is suitable for high-throughput analysis.

This paper introduces a bioengineered platform for bilaterally accessible GI organoid monolayers, enabling improved observability and studies of epithelial dynamics and pathogen interactions.

This study establishes thymic epithelial organoids that mimic in vivo thymus function and support T cell development in vitro.

This paper develops bioengineered human colon organoids that mimic in vivo complexity and function, enabling long-term study of gut physiology and disease.

This study reconstructs the colorectal cancer tumor microenvironment using patient-derived organoids and co-cultures, revealing how stromal cells influence cancer behavior and immune evasion.

This study develops an engineered uterus-like microenvironment to study mouse embryo implantation, revealing coordination between trophoblast migration and embryo growth.

This paper develops a genetic toolbox for tissue-derived human organoids, enabling efficient gene manipulation through CRISPR-based methods and flow cytometry.

This study develops EpiTS embryoids, which mimic post-implantation development in vitro by combining ESC and TSC aggregates to model gastrulation and axial morphogenesis.

This study develops a method to generate gastruloids with anterior neural tissues by inhibiting WNT signaling in mouse ESCs without exogenous Wnt activation.

This study uses gastruloids to model embryonic blood development, identifying blood progenitor cells and their spatial localization with spatiotemporal resolution.

This paper demonstrates that mouse embryonic stem cells can generate heart-like structures with in vivo-like morphogenesis, supporting cardiovascular progenitors and early heart field compartments.

This study uses Gastruloids to show that endoderm progenitors maintain epithelial identity and develop into distinct gut regions without extraembryonic tissues.

Mouse embryonic stem cell aggregates (Gastruloids) form anteroposterior, dorsoventral and left-right axes without extraembryonic tissue or localised signals, through Nodal-initiated, Wnt-stabilised T/Brachyury polarisation.