REALM: AO-based localization microscopy deep in complex tissue
M. E. Siemons, N. A. K. Hanemaaijer, M. H. P. Kole, L. C. Kapitein
Abstract
Performing Single-Molecule Localization Microscopy (SMLM) in complex biological tissues, where sample-induced aberrations hamper detection and localization, has remained a challenge. Here we establish REALM (Robust and Effective Adaptive Optics in Localization Microscopy), which corrects aberrations of [≤]1 rad RMS using 297 frames of blinking molecules to improve single-molecule localization. We demonstrate this method by resolving the periodic cytoskeleton of the axon initial segment at 50 m depth in brain tissue.
02 /Specification & Classification
Click any tag to browse matching papersReproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Label-free adaptive optics single-molecule localization microscopy for whole animals
- Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view
Isabel Droste, Erik Schuitema, … Bernd Rieger·OpticaPeer-reviewed·Jun 4, 2025
- Aberration correction for deformable mirror based remote focusing enables high-accuracy whole-cell super-resolution imaging
W. Shi, Y. He, … Y. Li·Photonics ResearchPeer-reviewed·Apr 1, 2024
- Aberration-aware 3D localization microscopy via self-supervised neural-physics learning
Shuang Fu, Wei Shi, … Yiming Li·Nature CommunicationsPeer-reviewed·May 16, 2026
- Improving localization precision via restricting biomolecule confined stochastic motion in SMLM
- Correlation Single Molecule Localization Microscopy (corrSMLM) Detects Fortunate Molecules for Better Localization Precision and High Signal-to-Noise Ratio
A. S, F. C. Zanacchi, P. P. Mondal·bioRxiv·Dec 29, 2022