cryo-EM

This study uses multiscale modeling and cryo-EM to show that transient protein structures guide electron transfer in mitochondrial supercomplexes, improving energy conversion efficiency by 30%.

This paper describes a laboratory's transition from X-ray crystallography to cryo-EM for studying ATAD2B, detailing challenges and strategies in sample preparation, data processing, and model building.

This study determines the cryo-EM structures of hSAMD9L and an N-terminal-truncated mutant, revealing their multi-domain architecture, dimerization, and nucleotide binding properties.

The paper investigates how the ultrastructure of biomolecular condensates, using PopZ as a model, influences their function through molecular interactions and phase-dependent conformational changes.

The paper determines cryo-EM structures of human IP3R-2 in different conformations and clusters, revealing ligand interactions and subtype-specific features.

This study presents the cryo-EM structure of human TWIK-2 and identifies key residues and modulators, providing a foundation for developing anti-inflammatory therapeutics.

This paper demonstrates a method to reduce sample consumption in time-resolved serial crystallography by using segmented droplet generation and mix-and-inject approaches for studying NQO1's redox cycle.

The paper describes a protein fold switch in a bactericidal nanomachine that enables membrane puncture and bactericidal activity.

The paper identifies how NS1619 binds to BK channels and stabilizes their open conformation through structural and dynamic analysis.

The paper designs rigid protein fiducials using generative design and deep learning to enable cryoEM visualization of GPCR conformational states, validated with four GPCR inactive structures.

The paper introduces cryoJAX, a cryo-EM image simulation library built on JAX for developing data analysis techniques in cryo-EM.

This study investigates how adenine nucleotides potentiate human type 2 IP3R activity by rigidifying the juxtamembrane domain through structural and computational analysis.

This study uses cryo-EM to reveal structural intermediates of CSN-mediated SCF deneddylation, providing insights into the mechanism and potential for drug design.

This study determines the structural basis of Arf1-driven membrane tubulation using cryo-EM, revealing how Arf1 forms curved tubules and accommodates cargo.

The paper identifies nucleotide codes that regulate dynein-Lis1 binding and reveal how Lis1 relieves dynein autoinhibition through structural changes.

This paper introduces ultrathin liquid cells for microsecond time-resolved cryo-EM, enabling longer observation windows and improved resolution for studying protein dynamics.

This paper reports the first high-resolution cryo-EM structures of an S9D protease, revealing its dimerization mechanism, catalytic regulation, and substrate selectivity through hinge-loop gating and conserved glutamate pocket.

This study determines the near-atomic structure of H. pylori sheathed flagella using cryo-EM, revealing how flagellins and glycans contribute to filament stability and motility.

This paper introduces genetically encoded protein tags with defined shapes for direct molecular identification by cryo-ET, demonstrating their effectiveness in identifying proteins in situ without disrupting native structures.

The paper characterizes the structure of a KLC2/Nup358/BicD2 complex using cryo-EM and SAXS, showing changes in stoichiometry and structure upon BicD2 binding.

The paper uses multimodal imaging to study the dynamic structure of the exocyst complex during exocytosis, revealing its conformational changes and tethering mechanism.

The paper determines the capsid structure of Atkinsonella hypoxylon virus using cryo-EM and identifies conserved and divergent features among partitiviruses.

This study investigates HIV-1 Env-MA interactions using cryo-ET and simulations, revealing the Kennedy-sequence motif's role in Env incorporation and Gag maturation's effect on Env clustering.

The paper determines the structure of the portal vertex and neck complex in the hyperthermophilic phage Oshimavirus, revealing a pressure-sensitive mechanism for terminating DNA packaging and triggering tail attachment.

This study shows that native electrospray ion beam deposition preserves membrane protein structure, enabling high-resolution cryo-EM analysis of complexes like AmtB.

The paper introduces a 3D-printed fixed-target platform for macromolecular crystallography that simplifies microcrystal handling and data collection at synchrotrons.

This study uses cryo-EM to reveal how lipid-mediated pH changes gate connexin-46/50 gap junctions, showing reversible structural transitions under acidic conditions.

The paper determines the cryo-EM structure of the Tap3-Tke5 complex, revealing the mechanism of adaptor-effector binding for a pore-forming toxin family.

This study identifies a hybrid Gag lattice as an intermediate in HIV-1 maturation, showing it is stabilized by IP6 and essential for infectivity.

This study reveals an unconventional activation mechanism of the atypical GPCR ACKR3 using engineered nanobodies and cryo-EM, showing that extracellular pocket volume changes, not microswitch conformational shifts, drive receptor activity.