cryo-EM

This study reveals conformational differences in HIV-1 Env glycoprotein on virions compared to soluble forms, highlighting the role of the viral membrane in maintaining native structure.

This study visualizes chromatin structure and HIV-1 nuclear trafficking in situ, showing HIV-1 cores preferentially associate with open chromatin and are excluded from compact regions.

The paper compares ribosome structures of naked mole-rats, tuco-tucos, and guinea pigs, finding fragmented 28S rRNA in the former with potential links to high translational fidelity.

The paper determines the cryo-EM structure of the hexameric MmpS4-MmpL4 complex from Mycobacterium tuberculosis, revealing the coiled-coil domain and conformational changes compared to the monomer.

This study reveals the structural basis of a phosphoinositide-driven mTORC2-AKT positive feedback loop through cryo-EM structures and functional analysis.

The paper determines the molecular structure of autophagic cargo condensates using cryo-electron tomography and shows how disordered regions influence condensate properties.

This study uses cryo-electron tomography to show temperature and receptor binding coordinate spike protein activation in HCoV-229E virions, revealing structural dynamics and conformational changes.

The paper reports the cryo-EM structure of the human P2X3 receptor complexed with sivopixant, revealing subtype-specific allosteric inhibition mechanisms and structural basis for improved selectivity.

The paper presents cryo-EM structures of type I and II Lamassu systems from different bacteria, revealing their architecture and mechanism of DNA cleavage.

The paper investigates the role of the quinol anion in respiratory complex I using simulations, showing its anionic nature and role in proton-coupled electron transfer.

This study determines the cryo-EM structures of TBC-DE and TBC-DEG assemblies to elucidate their mechanisms in regulating β-tubulin assembly and disassembly.

The paper determines the structure of a filamentous NONO/SFPQ heterooligomer using cryo-EM, revealing its organization and functional differences between the components.

The paper compares methods for analyzing heterogeneity in TRPV1 cryo-EM data and finds that different methods detect different types of heterogeneity.

The paper determines cryo-EM structures of tubulin cofactors bound to β-tubulin, revealing a vise-like mechanism for GTP-hydrolysis dependent α/β-tubulin biogenesis.

This study determines the structure of HSV-1 helicase-primase inhibited by HPIs and reveals how it interacts with the replication fork complex.

This paper introduces cryoFM, a generative foundation model for cryo-EM densities that improves reconstruction and refinement by combining data-driven priors with dataset-specific constraints.

This study determines the structural basis of cyclic phytocytokine recognition by HSL3/NUT receptor using cryo-EM, revealing a two-step recognition mechanism and the role of glycosylation in ligand binding.

The paper uses cryo-ET to show IFT trains dock at specific MTD positions in trypanosome flagella, revealing spatial regulation independent of IFT-B structure.

The paper presents an atomic structure of spinach rubisco with a transition-state analogue, revealing protonation dynamics and structural flexibility, suggesting new strategies for enzyme redesign.

The paper uses cryo-EM to capture non-equilibrium states of the μ-opioid receptor bound to different ligands, revealing ligand-dependent conformational dynamics and G-protein activation differences.

This study determines the cryo-EM structures of human ABCD3 in apo and substrate-bound states, revealing how substrate binding induces ATPase activity and provides structural insights into its transport mechanism.

This study investigates how disease-causing mutations and ivacaftor affect CFTR protein dynamics using molecular simulations and cryo-EM data.

Automated cryogenic plasma-focused ion beam milling and cryo-electron tomography of Chlamydomonas reinhardtii yield 1,829 annotated cellular tomograms and sub-nanometer in situ structures of six macromolecular complexes.

This study uses cryo-ET and sub-tomogram averaging to analyze Ty1 virus-like particles, revealing diverse capsomere arrangements and the role of CA-CTD flexibility in VLP heterogeneity.

The paper presents cryo-EM structures of classical pathway complement convertases and their substrate complex, revealing mechanisms of C3 cleavage and opsonin production.

This study uses cryo-ET and cryo-EM to reveal the molecular architecture and spatial organisation of proteasomes in human sperm nuclei, identifying unique features and their role in proteolysis.

This study determines the structure of TIRAP filaments and identifies the role of the N-terminal PBM in regulating their assembly and function at the plasma membrane.

The paper determines the cryo-EM structure of the IL-17RA, IL-17RB, and ACT1 complex, revealing how these proteins form a hetero-tetramer to initiate immune signaling.

The paper reports the first cryo-EM structure of ATTRv-F64S fibrils from a living patient's skin tissue, validating skin biopsy as a non-invasive diagnostic tool.

This study uses cryo-EM to determine the structure of LPOR-Chlide complexes, revealing a novel photocatalytic mechanism and structural basis for membrane remodeling.