cryo-EM

This study reports cryo-EM structures of the 50S ribosome with EngA-bound GTPγS or GDP, revealing conformational changes in EngA and a novel SLJ motif during ribosome assembly.

This study determines the atomic structure of mycobacteriophage SWU1 using cryo-EM, revealing key architectural features critical for virion assembly and infection.

The paper identifies bacterial MVP homologs and reports cryoEM structures of vaults from Corallococcus praedator and a Deltaproteobacteria bacterium, revealing novel symmetries.

The paper uses integrative modeling to reveal inter-domain flexibility in F-box proteins, showing varying conformational dynamics across different F-box proteins.

The paper reports the cryo-EM structures of an Acinetobacter baumannii podovirus, revealing its capsid maturation mechanism and providing a reference for phage engineering.

This study investigates the stepwise assembly and mechanical stability of nsP3 helical scaffolds in Chikungunya Virus using HS-AFM and EM, revealing domain-specific contributions to scaffold formation and function.

The paper identifies detergent LMNG as stabilizing a weak FZD-G protein interface, revealing that FZDs inherently weakly couple to G proteins and preferentially signal through DVL.

This study investigates how tau protein regulates motor proteins on microtubules using single-molecule imaging, cryo-EM, and MD simulations, revealing distinct mechanisms for inhibiting kinesin-1 and dynein.

The paper benchmarks cryo-EM re-refinement selection methods against no operation using a fixed cohort and scoring tools, finding increased disagreement with higher-resolution references.

This study identifies how LRRC58 senses cysteine through structural changes that regulate CDO1 binding and cysteine homeostasis.

This study presents cryo-EM structures of RtcB2 bound to damaged E. coli 30S subunits, revealing how the enzyme recognizes and repairs ribosomal RNA.

This study determines cryo-EM structures of FcR bound to IgM and IgA, revealing its binding mechanism and evolutionary insights into pIg receptor recognition.

Astex-Faro improves cryo-EM hit identification by detecting structural deviations and enhancing weak features across diverse datasets.

CryoID2 uses deep learning and sequence analysis to identify proteins and RNA from cryoEM maps, achieving high accuracy in molecular identification.

This study reports cryo-EM structures of human PAT-1 with inhibitors to elucidate their inhibition mechanisms and domain interactions.

The paper maps AMPA and NMDA receptors at mouse hippocampal synapses using cryo-ET, revealing their distribution and relationship to glutamate release sites.

The paper investigates how oligodendrocytes contribute to the formation of α-synuclein filaments in multiple system atrophy by comparing seeded aggregation in different cell types.

This study determines the cryo-EM structures of Cereibacter sphaeroides ferrochelatase, revealing oligomeric states that occlude the substrate-binding site.

The paper introduces a training-free method for particle picking and centering in cryo-EM by using a radial kernel convolution, achieving comparable reconstruction resolutions to reference-based methods.

This paper introduces an end-to-end reconstruction algorithm for cryo-ptycho single-particle analysis, achieving better resolution than sequential methods on simulated data and some experimental datasets.

This paper introduces cryoSHIELDs, tunable peptides that improve cryo-EM sample preparation by modulating the air-water interface, enhancing particle distribution and complex integrity across multiple protein systems.

The paper characterizes pseudaminic acid modifications on the lateral flagellar filament of Vibrio alginolyticus and shows Maf is required for filament formation.

This study identifies structural adaptability in HIV-2 Vif that enables broad antagonism of APOBEC3G through conformational selection.

This study determines the structural basis of MCAM-mediated inhibition of laminin-dependent cell migration by showing MCAM and integrin compete for a shared laminin interface.

This study uses cryo-EM to capture intermediate states of maltooligosaccharide transport by the SusCD complex in Bacteroides, revealing its heterodimeric structure and binding mechanisms.

This study uses cryo-EM and tomography to reveal the structural changes in bacteriophage P2 during tail contraction and host infection, identifying new architectural features and mechanisms of genome translocation.

The paper introduces a split-GFP graphene grid platform for selective protein capture in cryo-EM, demonstrating improved enrichment and a 2.58 Å cryoEM reconstruction of GFP11-tagged apoferritin.

The paper identifies tailocins as contaminants in ex vivo amyloid preparations and proposes strategies to detect and avoid them in cryo-EM.

The paper presents cryo-EM structures of the Rixosome and pre-60S particles to elucidate the dynamic architecture and activation mechanism of the Rixosome for ITS2 processing.