cryo-EM

The paper identifies a stress-induced membrane insertion mechanism that activates the BepA metalloprotease by displacing its autoinhibitory plug, linking membrane association to protease function.

The paper determines the in vivo structures of P22 infection intermediates and explains how gp10 templates the sequential assembly of three proteins into a trans-envelope channel for genome translocation.

The paper reports a cryo-EM structure of detergent-bound Vibrio cholerae MakA, revealing its conformational changes and role in pore assembly.

The paper uses computational and experimental methods to identify transition intermediates in the μ-opioid receptor-Gi protein complex that encode ligand efficacy.

This study presents cryo-EM structures of MBOAT2, revealing its catalytic mechanism, product retention, and ligand exchange dynamics.

The paper resolves the native structure of the Trichonympha centriole cartwheel using cryo-electron tomography, revealing a zigzag stacking pattern of SAS-6 tetramers and their role in structural stability and polarity.

The paper investigates the structural organization and assembly of the bacterial spore envelope in Clostridium sporogenes, revealing common principles of crystalline and disulphide-based self-assembly across distantly related bacteria.

This study uses cryo-EM to examine how antibodies against different PfCSP epitopes affect the structure and shedding of the circumsporozoite protein on Plasmodium falciparum sporozoites.

The paper determines the structure of HIV-1 capsid lattice bound to MxB N-terminal fragment, revealing an alpha-helix stabilizes binding at the trimer interface.

The paper reports a cryo-EM structure of a 95-basepair DNA minicircle at 5.3 Å resolution, revealing its B-DNA configuration and structural features.

The study uses molecular dynamics simulations to show that wild-type hERG fails to conduct K+ and evolves toward an inactivated-like conformation, contrasting with the N629D mutant which maintains conductivity.

This study reports cryo-EM structures of comammox AMO from Nitrospira inopinata and identifies its catalytic and inhibitor-responsive architecture, revealing a methyl-PQ-linked mechanism and stress response to nitrification inhibitors.

The paper reports cryo-EM structures of melanocortin receptors bound to endogenous inverse agonists, revealing mechanisms of inverse agonism and subtype selectivity.

This study identifies the RY12 domain of RyR1 as an ADP sensor and reveals dantrolene's binding mechanism through cryo-EM structures.

This paper develops a multimodal framework to align protein sequences with cryo-EM density maps using joint representation learning, achieving strong retrieval performance across species and resolutions.

The paper determines how MBOAT1 remodels phosphatidylserine by solving its cryo-EM structures and combining them with lipidomics and simulations.

The paper reports the cryoEM structure of the ITG3β1-CD151 complex and shows CD151 promotes ITG3β1 surface export, explaining the epidermolysis bullosa phenotype.

This study characterizes the structure and function of RvPrxL, a neofunctionalized peroxiredoxin from tardigrades that binds nucleic acids in the nucleus.

This paper develops a cryoprotectant-assisted plunge freezing method for preserving thick mouse brain tissue for cryo-imaging, enabling high-resolution visualization of cellular structures and validating their physiological relevance.

The paper investigates how BIM1 selectively inhibits CLC-Ka over CLC-Kb by analyzing cryo-EM structures and showing that interactions with a conserved lysine and dynamic gating-loop behavior underlie this selectivity.

The paper designs and characterizes triazole-based peptidomimetics of the -GI conotoxin, showing they retain potency and provide structural insights into their interaction with the muscle-type nAChR.

This study designs prefusion-stabilized HSV-2 gB antigens using structure-based methods and evaluates their immunogenicity, finding no improvement in neutralizing antibody titers compared to postfusion constructs.

The paper reports the cryo-EM structure of CYP2C9, revealing a dimer-of-trimers assembly and its stabilization mechanisms.

The paper determines the cryo-EM structure of Jabs, a bacteriophage infecting Mycobacterium abscessus, revealing unique capsid assembly and adhesion features.

This study determines the structures of the human NaCT protein in three states to elucidate the mechanism of sodium-substrate coupling in the DASS family.

The paper determines the structure of the DAB1 complex from Thermocrinis albus using cryo-EM, revealing how proton translocation couples to active-site remodeling for directional CO2 hydration.

The paper uses cryo-EM to analyze lambda-6 light chain amyloid structures from different organs and patients, revealing structural diversity and potential interactions with collagen-VI.

The paper determines the near-atomic structure of the P-SSP7 virion and identifies five rotational registers defining modular portal-capsid assembly.

The paper reports cryo-EM structures of GerA, revealing how nutrient binding triggers channel opening through allosteric switches and subunit interactions.