cryo-EM

This study reports the high-resolution structure of the native cardiac rigor cross-bridge, revealing how myosin heads and thin filament components coordinate during contraction.

The paper reports the cryo-EM structure of a blue-shifted PSI-LHCI-LHCII supercomplex from Posidonia oceanica, revealing structural adaptations for spectral diversification in higher plants.

This study uses cryo-EM to show that Factor XIa exists as a dynamic ensemble with flexible catalytic domains that expose the Factor IX-binding site.

The paper determines the Cryo-EM structure of a dodecameric double-ferritin-fold protein from an Asgard archaeon, revealing its unique structural features and implications for iron metabolism.

The paper reports cryo-EM structures of P. aeruginosa PA3214 and E. coli PqiC lipoproteins interacting with their MCE protein partners, revealing their assembly and functional interactions.

The paper identifies IPO9 as a regulator of cGAS-STING signaling by releasing cGAS from H2A-H2B dimer inhibition, revealing structural insights into this mechanism.

This study uses cryo-EM to analyze antibodies targeting HCMV glycoproteins, identifying synergistic combinations that enhance neutralization.

This paper introduces a method to reduce air-water interface artifacts in cryo-EM by encapsulating proteins in hydrophilic shells, demonstrating its effectiveness with three protein examples.

This study identifies lipoprotein-mediated endocytosis as a conserved mechanism for cationic cell-penetrating peptides across animal lineages, showing it modulates their antimicrobial function.

This study determines cryo-EM structures of orthoflaviviruses bound to LDLR family receptors, revealing distinct binding modes and receptor engagement mechanisms.

This paper investigates the activation mechanism of PP2A-B56δ by analyzing disease variant E198K using cryo-EM and computational methods, revealing progressive loosening of the N/C-arm interface as the activation pathway.

The paper presents cryo-EM structures of the delta opioid receptor with PAMs, identifies a novel allosteric site, and describes a mechanism for enhanced binding and activation.

The paper compares electrostatic properties of ATP synthase across species using crystallographic and cryo-EM structures, revealing species-dependent axial asymmetry in electrostatic potential.

The paper reports the cryo-EM structure of piscine orthoreovirus, showing structural conservation with mammalian orthoreoviruses despite low sequence identity.

This paper develops a Bayesian framework to model radiation damage in cryo-EM datasets by inferring atomic displacements and structural changes during electron irradiation.

The paper presents a cryo-EM structure of a euglenophyte PSII supercomplex and identifies LhcE9 as a far-red light-harvesting antenna subunit with unique structural and functional properties.

This study uses cryo-EM and computational methods to model the structure of HPV16 L2 protein within the capsid, revealing its conformational flexibility and positioning for virus entry.

This paper introduces ARCHER, a method for cross-specimen cryo-EM pose estimation that generalizes across structures using a contrastive classifier trained on protein structures.

This paper determines the cryo-EM structure of CbrA, revealing its SLC5-STAC domains and how CbrX interacts with the transporter, providing insights into histidine transport and signaling mechanisms.

This study investigates the assembly and conformational dynamics of insect odorant receptor complexes using high-speed atomic force microscopy and cryo-EM, revealing non-tetrameric oligomeric states and symmetry-broken configurations.

This paper presents an integrative structural analysis of the bacteriophage Ar-KM, revealing structural polymorphism and the role of ejection proteins in genome ejection.

The paper introduces cFAR and Relative Signal to diagnose preferred orientation in single-particle cryo-EM, using synthetic and real datasets to validate their effectiveness.

This study reveals that INO80 reorients on a nucleosome upon ATP hydrolysis, enabling DNA translocation through a unique mechanism distinct from other chromatin remodelers.

The paper compares RSV and hMPV entry strategies, showing RSV uses structural organization for thermotolerance while hMPV uses genomic redundancy for stability.

This study determines the structure of YcgO, an E. coli K+/H+ antiporter, and shows how unphosphorylated PtsN inhibits its activity through interactions with cytosolic domains.

The paper presents cryo-EM structures of bovine respiratory complex I under varying protonation conditions, revealing conformational changes at histidine junctions that coordinate proton translocation.

The paper describes a Kaggle challenge for particle picking in cryo-electron tomography, resulting in improved algorithms and highlighting the need for better annotation quality measures.

This study determines the cryo-EM structure of GPR30 with bicarbonate ions, identifies key residues for activation, and provides insights into G-protein coupling and drug development.

This study uses cryo-EM and charge detection mass spectrometry to determine the stoichiometry and structure of Bpa-substrate complexes in Mtb, revealing an undecameric apo form and a Bpa12:HspR2 complex.

This study determines the cryo-EM structure of MFSD2B bound to S1P and identifies its transport mechanism through mutagenesis and simulations.