Bacterial biofilms and quorum sensing

The paper characterizes the functional metabolic diversity of Pseudomonas aeruginosa clinical isolates by analyzing genotype-phenotype correlations and genome-scale metabolic networks.

This study shows Pseudomonas aeruginosa uses potassium efflux from human airway epithelial cells to enhance biofilm formation through the KdpFABCDE system.

This study shows Pf phages and sputum polymers reduce antibiotic diffusion through electrostatic binding and liquid crystal formation in chronic Pseudomonas aeruginosa infections.

This study evaluates the effectiveness of intermittent electrochemical generation of H2O2 and HOCl against MRSA biofilms, showing that HOCl treatment duration and sequencing significantly impact biofilm reduction.

This study develops a label-free method to detect spatial changes in c-di-GMP levels in microbial biofilms using MALDI-MSI and applies it to three bacterial species.

This study shows that mechanical interactions and spatial constraints in confined environments limit bacterial community diversity but allow stable heterogeneous populations, even with growth advantages.

This study identifies key regulatory factors and pathways affecting swarming motility in Pseudomonas fluorescens Pf0-1, showing that Gac/Rsm pathway activation enables swarming and that Gacamide A functions as a biosurfactant.

This study shows that increased mucin and eDNA concentrations in mucus enhance Pseudomonas aeruginosa antibiotic tolerance, and establishes an in vitro model to replicate this tolerance observed in sputum.

This study investigates how operational changes affect drinking water biofilm dynamics and their susceptibility to coliform invasion, finding that mature biofilms can be infiltrated by low concentrations of Serratia fonticola.

This paper develops a four-species polymicrobial biofilm model to study interspecies interactions in cystic fibrosis lung infections and observe clinically relevant phenotypes.

This study uses secondary metabolite profiling and whole-genome sequencing to identify rare genomic traits in Pseudomonas aeruginosa isolates that affect quorum sensing and metabolite biosynthesis.

This study shows that surface geometry influences genetic drift and selection in bacterial biofilms, with undulating surfaces creating stable clonal sectors that resist mixing and expansion.

This study shows that Staphylococcus aureus cells with lower buoyant density resist antibiotics more and form biofilms at air-liquid interfaces, while higher buoyant density cells are more antibiotic-sensitive and form biofilms on liquid-solid interfaces.

This study identifies a shared algal-bacterial extracellular matrix formed through bacterial exopolysaccharide production, enhancing aggregation and colonization in marine environments.

This paper identifies a bile-induced RND efflux pump in Bacteroides thetaiotaomicron that facilitates magnesium efflux, reducing eDNA concentration and promoting biofilm formation.

This study shows how four food preservatives exert distinct selective pressures on Salmonella Typhimurium planktonic and biofilm populations, leading to mutations in stress response regulators and trade-offs between biofilm formation and growth.

This paper uses LD-REIMS to show HOCl stress reduces PQS quorum sensing and virulence factors in P. aeruginosa.

This paper engineers two AHL acylases, PvdQ and MacQ, to improve their stability, activity, and substrate specificity for quorum quenching applications.

This paper identifies OprF and SigX as key factors in sub-MIC antibiotic-induced biofilm formation in Pseudomonas aeruginosa, linking periplasmic redox changes to c-di-GMP signaling.

This study shows that mexZ mutations in Pseudomonas aeruginosa lead to antibiotic tolerance and altered colonization in human airway models through overexpression of lecA.

This study reconstitutes a biofilm adhesin system from Desulfovibrio vulgaris in Pseudomonas fluorescens, showing that DvhA's N-terminal fragment is regulated by DvhG/DvhD and c-di-GMP levels.

The paper introduces iDynoMiCS 2.0, an individual-based modeling platform for microbial communities, showing that filamentous bacteria outcompete spherical bacteria regardless of growth strategy.

This paper shows that sphingosine induces PlcH transcription in Pseudomonas aeruginosa via SphR, through a promoter upstream of cerN and read-through of its terminator.

This study shows rhamnolipid micelles from Pseudomonas aeruginosa are highly cytotoxic to host and bacterial cells, correlating with virulence in clinical isolates.

This paper tests combination treatments to disrupt Pseudomonas aeruginosa biofilms in mucus, finding that NP40, hyaluronic acid, and tobramycin most effectively increase mucociliary transport and reduce bacterial viability.

This study uses transposon sequencing to identify Pseudomonas aeruginosa genes critical for colonization of Drosophila melanogaster, revealing conserved and host-specific factors.

This study shows that biofilm lifestyle selects for mutations in β-lactamases that reduce biofilm inhibition while maintaining antibiotic resistance.

This study identifies genes in Flavobacterium johnsoniae critical for surface colonization in both solitary and community settings using a model microbial community.