The paper measures the Young's modulus of single root hair cell walls using custom glass microplates as cantilevers to quantify mechanical properties.

bioRxiv · Dec 20, 2022Biophysics

Mechanical resistance of the environment affects root hair growth and nucleus dynamics

D. Pereira, T. Alline, E. Lin, A. Asnacios

This study shows that mechanical resistance affects root hair growth speed and nucleus movement, with stiffer environments producing shorter root hairs and altered nucleus positioning.

bioRxiv · Dec 15, 2022Biophysics

Shigella ipaA mediates actin bundling through diffusible vinculin oligomers with activation imprint

C. M. Valencia-Gallardo, D. I. Aguilar, H. Khakzad, B. Cocom-Chan, C. Bou-Nader, C. Velours, Y. Zarrouk, C. Le Clainche, C. Malosse, D. Borges-Lima, N. Quenech'Du, B. Mazhar, S. Essid, M. Fontecave, A. Asnacios, , G. Tran Van Nhieu

This study shows that Shigella IpaA induces vinculin homo-oligomerization, leading to actin bundling and stable cell adhesion independent of force transduction.

bioRxiv · Nov 7, 2022 · PreprintMicrobiology

Phenotyping Polarization Dynamics Of Immune Cells Using A Lipid Droplet - Cell Pairing Microfluidic Platform

L. Pinon, N. Ruyssen, J. Pineau, O. Mesdjian, D. Cuvelier, A. Chipont, R. Allena, C. Guerin, S. Asnacios, A. Asnacios, P. Pierobon, J. Fattaccioli

This study uses a microfluidic platform to analyze how surface properties affect B cell polarization during immune synapse formation.

bioRxiv · Dec 22, 2021Biophysics

Optineurin links Hace1-dependent Rac ubiquitylation to integrin-mediated mechanotransduction to control bacterial invasion and cell division

S. Petracchini, D. Hamaoui, A. Doye, A. Asnacios, F. Fage, E. Vitiello, m. Balland, S. Janel, F. Lafont, M. Gupta, B. Ladoux, J. Gilleron, T. M. Maia, F. Impens, L. Gagnoux, , A. Mettouchi

This study shows OPTN regulates bacterial invasion by linking HACE1-mediated Rac1 ubiquitylation to integrin mechanotransduction and ECM stiffness.

bioRxiv · Sep 19, 2021Cell Biology

Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads

A. SOUCHAUD, A. BOUTILLON, G. CHARRON, A. ASNACIOS, C. NOUS, N. B. DAVID, F. GRANER, F. Gallet

The paper develops a method to measure 3D shear stress in tissues using elastic beads and live imaging, demonstrating its application in cell aggregates and zebrafish embryos.

bioRxiv · May 10, 2021Biophysics

Intracellular stiffening competes with cortical softening in glioblastoma cells

C. Alibert, D. Pereira, N. Lardier, S. Etienne-Manneville, B. Goud, A. Asnacios, J.-B. Manneville

This study shows that glioblastoma cells are softer globally but stiffer intracellularly, suggesting mechanical phenotyping could be useful for diagnosis.

bioRxiv · Sep 2, 2020 · PreprintBiophysics

ipaA triggers vinculin oligomerization to strengthen cell adhesion during Shigella invasion

C. Valencia-Gallardo, D. I. Aguilar Salvador, H. Khazad, C. Bou-Nader, C. Malosse, D. Borges-Lima, C. Bello, B. Cocom-Chan, N. Quenech'Du, B. Mazhar, D. Javelaud, J. Fattaccioli, A. Mauviel, M. Fontecave, A. Asnacios, , G. Tran Van Nhieu

This paper shows that Shigella's IpaA protein causes vinculin to oligomerize, strengthening cell adhesion during bacterial invasion.

bioRxiv · Aug 14, 2020 · PreprintCell Biology