A. Skaist

Islands of genomic stability in the face of genetically unstable metastatic cancer

The paper identifies genomically stable regions in metastatic pancreatic cancer that can be targeted by CRISPR-Cas9 due to preserved PAM sites, suggesting a potential gene therapy approach.

Convergent alterations in the tumor microenvironment of MYC-driven human and murine prostate cancer

This study identifies MYC signaling activation as a common driver of tumor microenvironment alterations in human and murine prostate cancer through single-cell RNA-sequencing and comparative analysis.

CRISPR-Cas9 for selective targeting of somatic mutations in pancreatic cancers

This paper demonstrates CRISPR-Cas9 can selectively target somatic mutations in pancreatic cancer by exploiting PAMs in noncoding regions, achieving >80% cell death with minimal off-target effects.

Simultaneous CRISPR-Cas9-induced double strand breaks are lethal in models of pancreatic cancer

This study shows CRISPR-Cas9-induced DNA double-strand breaks are more cytotoxic to pancreatic cancer cells than radiation-induced breaks, with significant tumor growth inhibition and chromosomal instability.

Single-cell atlas of epithelial and stromal cell heterogeneity by lobe and strain in the mouse prostate

The paper constructs a single-cell atlas of mouse prostate cell types across strains and lobes, revealing strain- and lobe-specific transcriptomic differences in epithelial cells and strain-lobespecific fibroblast subtypes.

Phased Mutations and Complex Rearrangements in Human Prostate Cancer Cell Lines through Linked-Read Whole Genome Sequencing

This study uses linked-read whole genome sequencing to phase mutations and structural variants in prostate cancer cell lines, revealing gene-level haplotypes and complex rearrangements associated with castrate resistance.