X. Pan

PIN2-mediated self-organizing transient auxin flow contributes to auxin maxima at the tip of Arabidopsis cotyledons

The paper shows that PIN2-mediated transient auxin flow creates auxin maxima at Arabidopsis cotyledon tips, coordinating pavement cell interdigitation.

Development, validation and application of an LC-MS/MS method quantifying free forms of the micronutrients queuine and queuosine in human plasma using a surrogate matrix approach

The paper develops and validates an LC-MS/MS method to quantify free queuine and queuosine in human plasma, finding no detectable Q but significant q levels with no gender difference.

Urine Proteomics in Methamphetamine Addicted Patients

This study compares urine proteomes of methamphetamine users, withdrawers, and healthy individuals, identifying differentially expressed proteins linked to addiction and neurotoxicity.

Hierarchical global and local auxin signals coordinate cellular interdigitation in Arabidopsis

The paper shows auxin signaling coordinates cell shape in Arabidopsis through global and local pathways involving TIR1/AFB and TMK.

The 11β-hydroxysteroid dehydrogenase type 1 inhibitor SPI-62 prevents morbidity in a mouse model of Cushing's syndrome

SPI-62, an HSD-1 inhibitor, reduces GC-induced morbidity in a mouse Cushing's syndrome model by improving insulin sensitivity, reducing adiposity, and preventing skeletal myopathy.

Investigation of Aspergillus fumigatus small RNA biogenesis uncovers evidence of double-stranded RNA-dependent growth arrest.

This paper investigates small RNA biogenesis in Aspergillus fumigatus, revealing limited RNAi machinery involvement and identifying tRNA-derived RNAs linked to fungal growth arrest via dsRNA.

Secondary deficiency of neuraminidase 1 contributes to CNS pathology in neurological mucopolysaccharidoses via hypersialylation of brain glycoproteins

This study shows that reduced NEU1 activity in neurological MPS leads to hypersialylation of brain glycoproteins, contributing to CNS pathology through disrupted synaptogenesis and behavior.

The 3D ultrastructural morphology of a marine ammonia-oxidizing archaeon

This study uses cryo-ET and STEM-EDS to reveal the ultrastructural morphology and elemental composition of Nitrosopumilus maritimus SCM1, identifying two types of storage granules.

Distinct Roles of Kif6 and Kif9 in Mammalian Ciliary Trafficking and Motility

This study identifies distinct roles of Kif6 and Kif9 in mammalian ciliary trafficking and motility, showing Kif6 is a motile cilia-specific IFT motor while Kif9 regulates the central apparatus.

Tumor- and Nerve-Derived Axon Guidance Molecule Promotes Pancreatic Ductal Adenocarcinoma Progression and Metastasis through Macrophage Reprogramming

This study identifies SEMA3D as a dual driver of pancreatic ductal adenocarcinoma progression and metastasis through tumor- and nerve-derived mechanisms involving macrophage reprogramming.

A muscle hypertrophy-derived myokine reprograms the stromal vascular fractions differentiation towards thermogenic adipocytes in subcutaneous adipose tissue

This study identifies Thbs4 as a muscle-derived myokine that promotes the conversion of white to beige adipocytes in subcutaneous adipose tissue, improving metabolic health and protecting against high-fat diet-induced disorders.

The interferon/STAT1 signaling axis is a common feature of tumor-initiating cells in breast cancer

The paper identifies an IFN/STAT1 transcriptional state in tumor-initiating cells (TIC) of breast cancer using single-cell RNA sequencing, showing it differs from bulk RNA-seq derived TIC signatures.

CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing Loss

The paper develops a scalable microfluidic electroporation system to load gRNA:Cas9 RNP complexes into extracellular vesicles, achieving effective gene therapy for hearing loss in a mouse model.

Genome-wide Perturbation Analysis Screening System for Exosomes-Related Genes Based on the CRISPR/Cas9 Platform

The paper develops a CRISPR-UMI-based screening system to study exosome biogenesis, transport, and uptake mechanisms on a genome-wide scale.

Convergent alterations in the tumor microenvironment of MYC-driven human and murine prostate cancer

This study identifies MYC signaling activation as a common driver of tumor microenvironment alterations in human and murine prostate cancer through single-cell RNA-sequencing and comparative analysis.

Fungal-bacteria interactions provide shelter for bacteria in Caesarean section scar diverticulum

The paper investigates fungal-bacterial interactions in CSD, finding that fungal species alter bacterial abundance through metabolites, contributing to dysbiosis.

Abnormal homeostasis of P53 gene knockout mice can be reflected in urinary proteome

The paper analyzes the urinary proteome of p53 knockout mice to identify differential proteins and their associations with disease pathways.

Structural insights into ligand recognition and selectivity of the human hydroxycarboxylic acid receptor HCAR2

This study reports cryo-EM structures of HCAR2-Gi1 complexes with agonists, revealing ligand recognition, selectivity, and activation mechanisms for drug design.

Local administration of a novel siRNA modality into the CNS extends survival and improves motor function in the SOD1G93A mouse model for ALS

This study demonstrates that a novel siRNA modality, conjugated with an accessory oligonucleotide, improves survival and motor function in a mouse model of ALS when delivered locally to the CNS.

Changes in the urine proteome after massage in healthy people

This study identifies changes in the urine proteome of healthy individuals after massage, finding more significant differences at 2h compared to 24h post-massage.

Excess PrPC inhibits muscle cell differentiation via miRNA-enhanced liquid-liquid phase separation implicated in myopathy

Lhcb9-dependent photosystem I structure in moss reveals evolutionary adaptation to changing light conditions during aquatic-terrestrial transition

The paper reports the high-resolution structure of PpPSI-L in moss, revealing the role of Lhcb9 and phosphorylated LHCII in stabilizing the complex and its ability to switch between three types for light adaptation.

A stable reference human transcriptome and proteome as a standard for reproducible omics experiments

The paper establishes a stable human cell line as a reference for transcriptomic and proteomic studies, showing consistent results across generations and platforms.

A genetically encoded tool to increase cellular NADH/NAD+ ratio in living cells

This paper introduces a genetically encoded tool to increase the NADH/NAD+ ratio in mammalian cells and characterizes its metabolic and transcriptomic effects.

Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptome.

This study investigates the role of RNA interference in Aspergillus fumigatus by analyzing genetic variation and functional outcomes of RNAi component knockouts.

Skeletal muscle lineage is dispensable for appendage regeneration in axolotl

This study shows that skeletal muscle is not required for limb regeneration in axolotls, as Pax7 mutants regenerate limbs with normal patterns and cell composition.

Changes of urinary proteomic before and after QIV and COVID-19 vaccination

The paper examines urinary proteomic changes before and after QIV and COVID-19 vaccination, finding immune-related pathway enrichment and suggesting prior exposure to coronaviruses.

Dietary inclusion of nitrite-containing frankfurter exacerbates colorectal cancer pathology, alters metabolism and causes gut dybiosis in APCmin mice

This study shows that feeding APCmin mice nitrite-containing frankfurter increases gastrointestinal tumors, lipid peroxidation, and gut dysbiosis compared to nitrite-free diets.

Why SARS-CoV-2 Omicron variant is milder? A single high-frequency mutation of structural envelope protein matters.

The paper identifies a mutation in the SARS-CoV-2 Omicron variant's structural envelope protein that reduces ion channel calcium permeability and acid sensitivity, leading to lower cell damage and cytokine production.

CoVac501, a self-adjuvanting peptide vaccine conjugated with TLR7 agonists, against SARS-CoV-2 induces protective immunity

CoVac501, a self-adjuvanting peptide vaccine with TLR7 agonists, induces strong neutralizing antibodies and T cell responses in non-human primates against SARS-CoV-2.