This paper develops a CRISPR-Cas9-based system to manipulate O-GlcNAc modification at cis-regulatory elements, demonstrating its role in γ-globin gene regulation.

bioRxiv · Oct 27, 2022 · PreprintBiochemistry

Regulation of Liver Regeneration by hepatocyte O-GlcNAcylation in mice

D. R. Robarts, S. R. McGreal, D. S. Umbaugh, W. S. Parkes, M. Kotulkar, S. Abernathy, N. Lee, H. Jaeschke, S. Gunewardena, S. A. Whelan, J. A. Hanover, N. E. Zachara, C. Slawson, U. Apte

This study shows that O-GlcNAcylation in hepatocytes is essential for proper liver regeneration, with its loss leading to uncontrolled proliferation and tumor formation.

bioRxiv · Oct 14, 2021Pathology

O-GlcNAc modification of oncogenic transcription factor Sox2 promotes protein stability and regulates self-renewal in pancreatic cancer

N. Sharma, V. K. Gupta, P. Dauer, K. Kesh, R. Hadad, B. Giri, A. Chandra, V. Dudeja, C. Slawson, S. Banerjee, S. M. Vickers, A. Saluja, S. Banerjee

This study shows that O-GlcNAc modification of Sox2 stabilizes the protein and enhances self-renewal in pancreatic cancer, with targeting OGT reducing tumor burden.

bioRxiv · Jun 12, 2018Cancer Biology

O-GlcNAc Homeostasis Controls Cell Fate Decisions During Hematopoiesis

Z. Zhang, M. P. Parker, S. Graw, L. Novikova, H. Fedosyuk, J. Fontes, D. Koestler, K. Peterson, C. Slawson

This study shows that O-GlcNAc homeostasis regulates cell fate decisions in hematopoiesis by affecting transcriptional programs and differentiation outcomes.

bioRxiv · Jun 4, 2018Biochemistry

Elevated O-GlcNAcylation Enhances Pro-Inflammatory Th17 Function by Altering the Lipid Microenvironment

M. Machacek, Z. Zhang, E. P. Tan, J. Li, T. Li, M. T. Villar, A. Artigues, T. Lydic, C. Slawson, P. Fields

The paper shows that elevated O-GlcNAcylation in CD4+ T cells enhances Th17 function by altering the lipid microenvironment, increasing IL-17A production in obesity.

bioRxiv · Apr 20, 2018Immunology