D. Kern

The conformational landscape of fold-switcher KaiB is tuned to the circadian rhythm timescale

This paper characterizes the conformational landscape of KaiB, revealing its slow interconversion between states and how evolution tunes this process to match circadian rhythms.

Dual-Action Kinase Inhibitors Influence p38α MAP Kinase Dephosphorylation

The paper identifies dual-action kinase inhibitors that block the active site and enhance p38α dephosphorylation by stabilizing an accessible activation loop conformation.

Wide Transition-State Ensemble as Key Component for Enzyme Catalysis

The paper investigates the transition-state ensemble in adenylate kinase, finding a broad set of energetically equivalent configurations that support efficient enzyme catalysis.

A biophysical framework for double-drugging kinases

This study develops a biophysical framework to analyze double-drugging of kinases, revealing cooperative effects between orthosteric and allosteric inhibitors and providing structural insights.

From primordial clocks to circadian oscillators

This study investigates the primordial circadian clock in Rhodobacter sphaeroides using structural and kinetic analyses to elucidate its mechanism and evolutionary origins.

Prediction of multiple conformational states by combining sequence clustering with AlphaFold2

The paper uses sequence clustering with AlphaFold2 to predict conformational states of proteins and identifies mutations that switch between states.

Bimodal evolution of Src and Abl kinase substrate specificity revealed using mammalian cell extract as substrate pool

This study reveals the bimodal evolution of Src and Abl kinase substrate specificity using mammalian cell extract as a substrate pool, showing divergent evolutionary paths leading to specific and promiscuous enzymes.

Dynamics of human protein kinases linked to drug selectivity

The paper investigates whether protein dynamics after drug binding contribute to kinase inhibitor selectivity by analyzing Aurora A kinase interactions with three drugs.

Rescue of Conformational Dynamics in Enzyme Catalysis by Directed Evolution

This study uses directed evolution to identify mutations that restore catalytic activity in a mutant enzyme, revealing how conformational dynamics are shaped by evolutionary selection.

Astrin-SKAP complex reconstitution reveals its kinetochore interaction with microtubule-bound Ndc80

The paper reconstitutes the Astrin-SKAP complex and shows it synergizes with the Ndc80 complex to stabilize kinetochore-microtubule interactions.