This study uses single-molecule imaging to show how MRN and TOP3A-RMI1/2 coordinate with BLM and DNA2 to efficiently resect DNA during homologous recombination.

bioRxiv · Jul 1, 2022 · PreprintBiophysics

Massively Parallel Selection of NanoCluster Beacons

Y.-A. Kuo, C. Jung, Y.-A. Chen, H.-C. Kuo, O. S. Zhao, T. D. Nguyen, J. R. Rybarski, S. Hong, Y.-I. Chen, D. C. Wylie, J. A. Hawkins, J. N. Walker, S. W. Shields, J. S. Brodbelt, J. T. Petty, , H.-C. Yeh

The paper develops a high-throughput method to screen and design multicolor NanoCluster Beacons using NGS chips and machine learning.

bioRxiv · Dec 4, 2021Bioengineering

Rapid characterization of spike variants via mammalian cell surface display

K. Javanmardi, C.-W. Chou, C. I. Terrace, A. Annapareddy, T. S. Kaoud, Q. Guo, J. Lutgens, H. Zorkic, A. P. Horton, E. C. Gardner, G. Nguyen, D. R. Boutz, J. Goike, W. N. Voss, H.-C. Kuo, , I. J. Finkelstein

This study characterizes SARS-CoV-2 spike variants by assessing their expression, ACE2 binding, and neutralizing antibody recognition across multiple variants.

bioRxiv · Mar 30, 2021Biochemistry

Courtship ritual of male and female nuclei during fertilisation in Neurospora crassa

S. Brun, H.-C. Kuo, C. E. Jeffree, D. D. Thomson, N. Read

This study tracks male and female nuclei during fertilization in Neurospora crassa using live-cell imaging, revealing novel dynamics of nuclear movement and polyspermy avoidance.

bioRxiv · Mar 10, 2021Cell Biology

Structure-based Design of Prefusion-stabilized SARS-CoV-2 Spikes

C.-L. Hsieh, J. A. Goldsmith, J. M. Schaub, A. M. DiVenere, H.-C. Kuo, K. Javanmardi, K. C. Le, D. Wrapp, A. G.-W. Lee, Y. Liu, C.-W. Chou, P. O. Byrne, C. K. Hjorth, N. V. Johnson, J. Ludes-Meyers, , J. S. McLellan

This study designs and characterizes prefusion-stabilized SARS-CoV-2 spike variants to improve protein yield and stability for vaccine and diagnostic development.

bioRxiv · May 30, 2020Microbiology