J. E. Toettcher

This paper introduces pYtags, a tool for live-cell monitoring of specific RTK activity, showing it can track signaling dynamics and enable multiplexed reporting of multiple RTKs.

The paper uses an optogenetic system to show that clustering of Zap70 and LAT enhances downstream signaling compared to dimers, revealing a positive feedback loop involving Src-family kinases.

The paper quantifies how Erk signaling dynamics influence immediate-early gene expression in cells and shows that response heterogeneity is not fully explained by Erk activity.

This paper introduces a spatiotemporal model of dynamic cell signaling using Hawkes processes to capture both single-cell and spatial signaling behaviors in multi-cellular systems.

This paper introduces light-responsive monobodies (OptoMBs) that can reversibly control protein binding affinity, demonstrating their utility in purification and potential for light-switchable applications.

This study investigates the structural dynamics of the LOV2 domain in AsLOV2, showing that Q513 and N414 mediate J-helix unfolding linked to optogenetic function.

This paper develops opto-nanobodies (OptoNBs) that enable light-switchable binding to target proteins, showing applications in modulating signaling and protein purification.

The paper performs a live-cell screen of kinase inhibitors to identify modulators of Erk dynamics and shows how these dynamics affect cell proliferation.

The paper investigates size control in yeast by blocking bud initiation, showing that size regulation occurs through a timer rather than a molecular sensor.

This study maps intracellular phase separation behavior using an optogenetic system, showing localized phase separation can occur through diffusive capture of protein ligands.