Author

J. M. Mathiesen

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 2 Biophysics· 2 Pharmacology & Toxicology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Research Focus:cell signaling & trafficking· 2 Research Focus:drug response / drug discovery· 2 Research Focus:host-pathogen & immune response· 2 Organism:human· 2 Approach:biophysical modeling· 1 Research Focus:cell type annotation· 1 Research Focus:chromatin & epigenetic regulation· 1

Frequent Co-Authors

B. K. Kobilka· 3 shared K. Krishna Kumar· 3 shared D. Hilger· 2 shared H. Wang· 2 shared M. F. Pedersen· 2 shared A. Dias· 1 shared A. El Daibani· 1 shared B. White· 1 shared

Activity by Year

2019-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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3 shared papersBiochemistry

B. K. Kobilka

Mutual co-authors in cluster

Author profile →

5 papers

Recent·Most cited

Negative allosteric modulation of the μ-opioid receptor

E. S. O'Brien, V. A. Rangari, A. El Daibani, S. O. Eans, B. White, H. Wang, Y. Shiimura, K. Krishna Kumar, K. Appourchaux, W. Huang, C. Zhang, J. M. Mathiesen, T. Che, J. P. McLaughlin, S. Majumdar, B. K. Kobilka

This paper identifies a negative allosteric modulator of the μ-opioid receptor that blocks agonist activity and enhances naloxone efficacy, offering potential for safer opioid overdose reversal.

bioRxiv · Sep 8, 2023 · PreprintBiochemistry

Negative allosteric modulation of the glucagon receptor by RAMP2

K. Krishna Kumar, E. O'Brien, C. Habrian, N. R. Latorraca, H. Wang, I. Tuneew, E. Montabana, S. Marqusee, D. Hilger, E. Y. Isacoff, J. M. Mathiesen, B. K. Kobilka

This study shows RAMP2 inhibits GCGR signaling by inducing conformational changes and disorder in the receptor's extracellular domain.

bioRxiv · Aug 30, 2022 · PreprintBiochemistry

Suitable use of FRET-based Biosensors for Quantitative Detection of GPCR Activation

N. K. Brinkenfeldt, A. Dias, J. M. Mathiesen, K. L. Martinez

The paper quantifies cAMP production using a genetically encoded biosensor in living cells and shows that biosensor expression levels affect signal linearity, necessitating stable cell lines for accurate high-throughput screening.

bioRxiv · Jun 1, 2022 · PreprintBiophysics

Dissecting the roles of GRK2 and GRK3 in μ-opioid receptor internalization and β-arrestin2 recruitment using CRISPR/Cas9-edited HEK293 cells

T. C. Moller, M. F. Pedersen, J. R. van Senten, S. D. Seiersen, J. M. Mathiesen, M. Bouvier, H. Bräuner-Osborne

This study uses CRISPR/Cas9 to knockout GRK2 and GRK3 in HEK293 cells to assess their roles in μ-opioid receptor internalization and β-arrestin2 recruitment, finding GRK2 more critical than GRK3.

bioRxiv · Jan 8, 2020Pharmacology & Toxicology

Structural insights into ligand efficacy and activation of the glucagon receptor

D. Hilger, K. Krishna Kumar, H. Hu, M. F. Pedersen, L. Giehm, J. M. Mathiesen, G. Skiniotis, B. K. Kobilka

The paper presents the cryo-EM structure of the glucagon receptor bound to a ligand and G-protein, revealing structural insights into ligand efficacy and activation mechanism.

bioRxiv · Jun 5, 2019 · PreprintBiophysics