T. Che

Cryo-EM structures of human cone visual pigments

This study presents cryo-EM structures of human cone visual pigments in complex with G proteins, revealing their active state and mutations affecting spectral tuning.

Negative allosteric modulation of the μ-opioid receptor

This paper identifies a negative allosteric modulator of the μ-opioid receptor that blocks agonist activity and enhances naloxone efficacy, offering potential for safer opioid overdose reversal.

Conformational dynamics of the μ-opioid receptor determine ligand intrinsic efficacy

The paper uses biophysical techniques to show how ligand-induced conformational changes in the μ-opioid receptor affect its signaling efficacy.

Single cell Transcriptome and T cell Repertoire Mapping of the Mechanistic Signatures and T cell Trajectories Contributing to Vascular and Dermal Manifestations of Behcet Disease

This study maps single-cell transcriptomes and T cell repertoires in Behcet's disease to identify immune mechanisms and T cell trajectories in vascular and dermal manifestations.

Structural insights into distinct signaling profiles of the μOR activated by diverse agonists

This study compares the structural and signaling differences between two μOR agonists, lofentanil and mitragynine pseudoindoxyl, revealing how their distinct binding modes lead to different G protein and β-arrestin activation profiles.

Structure Determination of Inactive-State GPCRs with a Universal Nanobody

This paper uses nanobodies to determine cryo-EM structures of inactive-state GPCRs, achieving resolutions comparable to X-ray crystallography and providing insights into ligand binding.

A novel mitragynine analog with low efficacy mu-opioid receptor agonism displays antinociception with attenuated adverse effects

The paper develops a novel mitragynine analog with reduced mu-opioid receptor efficacy and adverse effects, showing antinociception without respiratory depression or hyperlocomotion.