J. Pacheco

Molecular mechanism for regulating APOBEC3G DNA editing function by the non-catalytic domain

This study identifies how AA/GA dinucleotides downstream of target-C sites enhance APOBEC3G DNA editing through structural and biochemical analysis of rhesus macaque A3G.

Missense mutations in the calcium-activated chloride channel TMEM16A promote tumor growth by activating oncogenic signaling in Human Cancer.

This paper investigates TMEM16A missense mutations in cancer, finding they promote tumor growth through altered signaling, independent of chloride flux.

FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity

FALCON systematically analyzes 61 free fatty acids to identify lipotoxic effects and a novel mediator of lipotoxicity, CMIP, in human pancreatic beta cells.

A Novel Homeostatic Mechanism Tunes PI(4,5)P2-dependent Signaling at the Plasma Membrane

The paper identifies PIP4K as a sensor and negative regulator of PI(4,5)P2 synthesis, revealing a homeostatic mechanism that tunes membrane functions.

Free diffusion of PI(4,5)P2 in the plasma membrane in the presence of high density effector protein complexes

The paper measures PI(4,5)P2 diffusion in the plasma membrane and finds that it diffuses rapidly even in regions with high effector protein density, suggesting free diffusion rather than spatial segregation.

Phosphatidylinositol 3-kinase-independent synthesis of PtdIns(3,4)P2 by a phosphotransferase

The paper shows that Salmonella and Shigella effectors generate PtdIns(3,4)P2 independently of phosphoinositide 3-kinases using a phosphotransferase mechanism.

Vitamin D Sufficiency Enhances Differentiation of Patient-Derived Prostate Epithelial Organoids

This study shows that vitamin D sufficiency promotes differentiation of prostate epithelial organoids by inhibiting Wnt signaling, linking vitamin D deficiency to aggressive prostate cancer.

Co-occurring alterations in the RAS-MAPK pathway limit response to MET inhibitor treatment in MET exon 14 skipping mutation positive lung cancer

The paper identifies co-occurring RAS-MAPK pathway alterations in MET exon 14 skipping mutation positive lung cancers that limit response to MET inhibitors and suggests combining MET and MEK inhibitors to overcome resistance.

A High Avidity Biosensor Reveals PI(3,4)P2 is Predominantly a Class I PI3K Signaling Product

The paper develops a high-avidity biosensor for PI(3,4)P2, revealing it is mainly produced by class I PI3K and hydrolyzed by PTEN.