S. Grinstein

Reactive oxygen species suppress phagocyte surveillance by oxidizing cytoskeletal regulators

This study shows that reactive oxygen species (ROS) from IgG-opsonized targets suppress phagocyte surveillance by oxidizing cytoskeletal regulators, leading to cell compaction and podosome disassembly.

Adenomatous polyposis coli (APC) regulates internalization and signaling of the chemorepellent receptor, Roundabout (ROBO) 1

This study identifies APC's role in SLIT2-induced ROBO1 endocytosis and its impact on cell morphology.

Endocytosis is regulated through the pH-dependent phosphorylation of Rab GTPases by Parkinson's kinase LRRK2

This study shows that LRRK2 kinase phosphorylates Rab GTPases in response to pH changes, affecting endocytic vacuole formation and vesicular traffic.

The chemorepellent, SLIT2, bolsters innate immunity against Staphylococcus aureus

SLIT2, a neuro-repellent, enhances neutrophil antibacterial activity against S. aureus by boosting reactive oxygen species production and granule exocytosis, improving infection clearance.

Lipid peroxidation increases membrane tension, Piezo1 gating and cation permeability to execute ferroptosis

This study shows that lipid peroxidation during ferroptosis increases membrane tension, activates Piezo1 and TRP channels, and causes cation permeability, leading to cell death.

Lysosomal swelling and lysis mediate delivery of C3 toxins to their cytoplasmic targets

This study investigates how C3 toxins enter host cells by tracking fluorescently labeled C3larvinA and shows that lysosomal swelling and lysis are critical for toxin delivery to the cytoplasm.

Detection and quantification of the vacuolar H ATPase using the Legionella effector protein SidK

The paper introduces a new probe derived from SidK to specifically localize and quantify V-ATPases in live and fixed cells, showing their progressive acquisition during phagosome maturation and subdomain distribution.

CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7

The paper uses cryoEM to study endogenous V-ATPase interactions with mEAK-7 in porcine kidney, revealing structural insights and functional differences compared to yeast.

Endothelial integration of mechanosensory signals by the spectrin cytoskeleton

This study shows that the spectrin cytoskeleton in endothelial cells is essential for sensing and transmitting shear stress to mechanosensors, leading to vasoactive responses.

Phosphatidylinositol 3-kinase-independent synthesis of PtdIns(3,4)P2 by a phosphotransferase

The paper shows that Salmonella and Shigella effectors generate PtdIns(3,4)P2 independently of phosphoinositide 3-kinases using a phosphotransferase mechanism.

Rab5 regulates macropinosome closure by recruitment of OCRL/Inpp5b and hydrolysis of PtdIns(4,5)P2

This study identifies Rab5's role in macropinosome closure by recruiting OCRL/Inpp5b and hydrolyzing PtdIns(4,5)P2.

Phosphatidylinositol-4-kinase IIα licenses phagosomes for TLR4 signaling and MHC-II presentation in dendritic cells

This study identifies PI4KII as essential for phagosomal TLR4 signaling and MHC-II presentation in dendritic cells by facilitating TIRAP recruitment.

Combined Atomic Force Microscope and Volumetric Light Sheet System for Mechanobiology

The paper introduces a combined AFM and light sheet microscope for mechanobiology, enabling high-resolution imaging and force measurements of cellular processes.

Candida albicans hyphal expansion causes phagosomal membrane damage and luminal alkalinization

This study shows that Candida albicans hyphal expansion causes phagosomal membrane damage and alkalinization, challenging the previous hypothesis that ammonia production drives phagosomal alkalinization.