L. E. Pedersen

Identification of hyperosmotic stress-responsive genes in Chinese hamster ovary cells via genome-wide virus-free CRISPR/Cas9 screening

The paper identifies hyperosmotic stress-responsive genes in CHO cells using CRISPR/Cas9 screening and validates their role in stress resistance and protein production.

A synthetic RNA-mediated evolution system in yeast

This paper introduces CRAIDE, a method using RNA donors and CRISPR to evolve yeast genomes more efficiently than traditional methods.

Synergistic stabilization of a double mutant in CI2 from an in-cell library screen

The paper identifies two stabilizing variants of barley chymotrypsin inhibitor 2, showing synergistic effects in a double mutant.

A dual-reporter system for investigating and optimizing protein translation and folding in E. coli

This paper introduces a dual-reporter system to assess protein translation and folding in E. coli, validated on five proteins with good correlation between reporter signals and protein expression/solubility.

A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes

The paper uses a CRISPR-Cas9 screen in Chinese hamster ovary cells to identify glutamine-sensitive genes, revealing a novel lipase involved in TCA cycle regulation.

Multiplex secretome engineering enhances recombinant protein production and purity

This study shows that deleting multiple host cell proteins in CHO cells improves recombinant protein production and purity through enhanced purification efficiency.

Adaptive laboratory evolution reveals general and specific chemical tolerance mechanisms and enhances biochemical production

This study uses adaptive laboratory evolution to identify chemical tolerance mechanisms in E. coli and shows how pre-tolerized strains can enhance biochemical production.