K. Teilum

A suicidal and extensively disordered luciferase with a bright luminescence

This paper investigates the structure and mechanism of Gaussia luciferase, revealing its disordered structure and identifying key residues for light production and inactivation.

Slow conformational changes in the rigid and highly stable chymotrypsin inhibitor 2

The paper investigates how mutations affect slow conformational dynamics and stability in chymotrypsin inhibitor 2, finding that the excited state population correlates with protein stability.

Mass spectrometry of RNA-binding proteins during liquid-liquid phase separation reveals distinct assembly mechanisms and droplet architectures

The paper uses mass spectrometry and simulations to study phase separation of RNA-binding proteins, revealing distinct assembly mechanisms and droplet structures.

Double mutant of chymotrypsin inhibitor 2 stabilized through increased conformational entropy

The paper investigates how mutations in CI2 affect conformational dynamics and stability, finding that increased native state entropy correlates with enhanced stability in double mutants.

Disease-linked mutations trigger exposure of a protein quality control degron in the DHFR protein

The paper identifies two PQC degrons in DHFR that become exposed in disease-linked missense variants, leading to proteasomal degradation.

Bi-directional protein-protein interactions control liquid-liquid phase separation of PSD-95 and its interaction partners

This study identifies the minimal peptide responsible for stargazin-mediated LLPS of PSD-95 and shows that pharmacological inhibitors can promote LLPS formation.

Synergistic stabilization of a double mutant in CI2 from an in-cell library screen

The paper identifies two stabilizing variants of barley chymotrypsin inhibitor 2, showing synergistic effects in a double mutant.

A dual-reporter system for investigating and optimizing protein translation and folding in E. coli

This paper introduces a dual-reporter system to assess protein translation and folding in E. coli, validated on five proteins with good correlation between reporter signals and protein expression/solubility.

Fitting side-chain NMR relaxation data using molecular simulations

The paper integrates side-chain NMR relaxation data with molecular dynamics simulations to improve the accuracy of protein dynamics models.

Charge Interactions in a Highly Charge-depleted Protein

This study investigates charge interactions in a charge-depleted protein using simulations and experiments, revealing context-dependent residue interactions and their impact on protein stability.

Global analysis of protein stability by temperature and chemical denaturation

This paper introduces a method to assess protein stability using combined temperature and denaturant unfolding with minimal protein requirements, demonstrating its applicability for small-scale protein purification.

Molecular dynamics ensemble refinement of the heterogeneous native state of NCBD using chemical shifts and NOEs

This study refines the native state ensemble of NCBD using chemical shifts and NOEs to accurately model its conformational dynamics.