N. G. Brown

This study shows that multi-monoubiquitination of VASP at specific lysine residues negatively regulates its actin-binding and bundling activities, affecting cell spreading morphology.

This paper describes a targeted protein degradation strategy using UNC8732 to recruit FBXO22 for NSD2 degradation, leading to growth suppression and reversal of drug resistance in leukemia cells.

The paper uses proteomics and PLK1 inhibition to identify proteins degraded at G2/M, showing PLK1's role in coordinating proteolysis via SCF ligases and linking AKAP2 to cell cycle control.

The paper presents the cryo-EM structure of UBR5, revealing its dimeric architecture and dynamic domain movements, and identifies AKIRIN2 as an interacting protein.

This study identifies cyclin F as a regulator of p130/RBL2 degradation via SCF, showing its role in cell cycle control and potential implications in cancer.

The paper predicts APC/C substrates in silico, identifies chromatin regulators as substrates, and shows their cell cycle-dependent degradation and impact on DNA methylation.

This paper demonstrates that mass photometry accurately quantifies sample heterogeneity with high resolution and minimal volume, outperforming negative stain electron microscopy in detecting proteasome assemblies.

The paper identifies a cryptic ubiquitin-binding site on APC/C using ubiquitin variants, revealing its preference for K48-linked chains and demonstrating a method for uncovering such sites in large complexes.