O. Zhang

Investigating 3D microbial community dynamics in the rhizosphere using complex-field and fluorescence microscopy

This paper introduces CFAST, a novel imaging technique for 3D microbial community dynamics in the rhizosphere, enabling non-invasive tracking of bacterial behavior and gene expression without genetic labeling.

Resolving the nanoscale structure of β-sheet assemblies using single-molecule orientation-localization microscopy

This study uses single-molecule orientation-localization microscopy to analyze the structure and polymorphism of engineered and pathological cross-beta fibrils.

Local Disordered Region Sampling (LDRS) for Ensemble Modeling of Proteins with Experimentally Undetermined or Low Confidence Prediction Segments

The paper introduces LDRS, a tool for generating conformational ensembles of intrinsically disordered regions in proteins, improving structural modeling of flexible segments.

POLCAM: Instant molecular orientation microscopy for the life sciences

POLCAM provides a fast, simplified method for single-molecule orientation microscopy using polarization cameras, enabling rapid molecular anisotropy analysis in biological samples.

Six-Dimensional Single-Molecule Imaging with Isotropic Resolution using a Multi-View Reflector Microscope

The paper introduces a raMVR microscope that achieves 6D super-resolution imaging of single molecules with high precision in 3D position and orientation.

IDPConformerGenerator: A Flexible Software Suite for Sampling Conformational Space of Disordered Protein States

IDPConformerGenerator generates diverse ensembles of disordered protein states using physical restraints and machine learning, enabling structural analysis of intrinsically disordered proteins.

Resolving the 3D rotational and translational dynamics of single molecules using radially and azimuthally polarized fluorescence

This paper introduces a radially and azimuthally polarized microscope to measure single-molecule orientation and dynamics with high precision, revealing cholesterol-induced changes in membrane behavior.

Single-molecule 3D orientation imaging reveals nanoscale compositional heterogeneity in lipid membranes

The paper introduces SMOLM, a single-molecule orientation localization microscopy technique, to measure orientation and spatial organization of lipophilic probes in lipid membranes, revealing compositional heterogeneity.

Single-molecule orientation localization microscopy for resolving structural heterogeneities between amyloid fibrils

The paper introduces a new performance metric, VUB, to efficiently evaluate orientation measurement performance of PSFs and applies it to study structural heterogeneities in amyloid fibrils using single-molecule orientation localization microscopy.

Structural basis of Toxoplasma gondii Perforin-Like Protein 1 membrane interaction and activity during egress

This study identifies the structural basis of TgPLP1 membrane interaction and its role in parasite egress by showing a hydrophobic loop insertion into the membrane is essential for cytolytic activity.