Author

L.-M. Needham

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Biophysics· 4 Biochemistry· 1 Cell Biology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 4 Research Focus:cell type annotation· 3 Organism:human· 3 Assay:fluorescence / live-cell microscopy· 2 Research Focus:host-pathogen & immune response· 2 Approach:microscopy & imaging analysis· 2 Assay:proteomics· 2 Research Focus:cell signaling & trafficking· 1

Frequent Co-Authors

D. Klenerman· 4 shared S. F. Lee· 3 shared A. Ponjavic· 2 shared S. J. Davis· 2 shared S. Lee· 2 shared A. Aubert· 1 shared A. Bruzas· 1 shared A. Carr· 1 shared

Activity by Year

2019-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

4 shared papersBiophysics

D. Klenerman

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

Label-free observation of individual solution phase molecules

L.-M. Needham, C. Saavedra, J. K. Rasch, D. Sole-Barber, B. S. Schweitzer, A. J. Fairhall, C. H. Vollbrecht, B. Mehlenbacher, Z. Zhang, L. Tenbrake, H. Pfeifer, E. R. Chapman, R. H. Goldsmith

This paper introduces a label-free technique to detect individual solution-phase molecules using microcavities, achieving high signal-to-noise ratios and resolving molecular radius and isomer mixtures.

bioRxiv · Mar 24, 2023Biophysics

POLCAM: Instant molecular orientation microscopy for the life sciences

E. Bruggeman, O. Zhang, L.-M. Needham, M. Koerbel, S. Daly, M. Cheetham, R. Peters, T. Wu, A. S. Klymchenko, S. J. Davis, E. K. Paluch, D. Klenerman, M. D. Lew, K. O'Holleran, S. F. Lee

POLCAM provides a fast, simplified method for single-molecule orientation microscopy using polarization cameras, enabling rapid molecular anisotropy analysis in biological samples.

bioRxiv · Feb 7, 2023Biophysics

Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, while the Alzheimer's R47H mutant does the opposite

A. Vilalta, Y. Zhou, J. Sevalle, J. K. Griffin, K. Sato, D. H. Allendorf, S. De, M. Puigdellivol, A. Bruzas, M. A. Burguillos, R. B. Dodd, F. Chen, Y. Zhang, P. Flagmeier, L.-M. Needham, , G. C. Brown

The paper shows wild-type sTREM2 inhibits Aβ aggregation and neurotoxicity, while the R47H variant promotes Aβ aggregation and neurotoxicity.

bioRxiv · Dec 3, 2020Biochemistry

Live-cell 3D single-molecule tracking reveals how NuRD modulates enhancer dynamics

S. Basu, O. Shukron, D. Hall, P. Parruto, A. Ponjavic, D. Shah, W. Boucher, D. Lando, W. Zhang, N. Reynolds, L. H. Sober, A. Jartseva, R. Ragheb, X. Ma, J. Cramard, , E. D. Laue

This study uses live-cell 3D single-molecule tracking to show that the NuRD complex decompacts chromatin and alters enhancer dynamics by increasing their movement and reducing time in a fast-diffusing state.

bioRxiv · Apr 3, 2020 · PreprintCell Biology

vLUME: 3D Virtual Reality for Single-molecule Localization Microscopy

A. Spark, A. Kitching, D. Esteban-Ferrer, A. Handa, A. R. Carr, L.-M. Needham, A. Ponjavic, M. Da Cunha Santos, J. McColl, C. Leterrier, S. J. Davis, R. Henriques, S. F. Lee

vLUME is a VR software for visualizing 3D SMLM data, enabling segmentation and quantification of fluorescence puncta with high fidelity.

bioRxiv · Jan 20, 2020Biophysics

ThX - A next-generation probe for the early detection of amyloid aggregates

L.-M. Needham, J. Weber, J. A. Varela, J. W. B. Fyfe, D. T. Do, C. K. Xu, B. Keenlyside, R. Cliffe, D. Klenerman, C. M. Dobson, C. A. Hunter, S. E. Bohndiek, T. N. Snaddon, S. F. Lee

This paper introduces ThX, a new fluorescent probe for detecting amyloid aggregates, showing improved brightness and binding affinity over ThT.

bioRxiv · Jul 24, 2019Biophysics