D. Klenerman

The delayed kinetics of Myddosome formation explains why Aβ aggregates trigger TLR4 less efficiently than LPS

This study shows that Aβ aggregates trigger TLR4 less efficiently than LPS due to slower Myddosome formation and longer lifetimes of larger Myddosomes.

Characterization and super-resolution imaging of small tau aggregates in human samples.

The paper develops a method to detect and characterize small tau aggregates in human samples, revealing disease-specific morphological and compositional differences.

High-density volumetric super-resolution microscopy

This paper introduces SMLFM, a volumetric super-resolution microscopy technique that achieves higher speed and accuracy in detecting single molecules in live cells.

POLCAM: Instant molecular orientation microscopy for the life sciences

POLCAM provides a fast, simplified method for single-molecule orientation microscopy using polarization cameras, enabling rapid molecular anisotropy analysis in biological samples.

Solution structure and synaptic analyses reveal determinants of bispecific T cell engager potency

The paper evaluates TcE formats to determine how contact distance and flexibility affect their potency in forming immunological synapses.

Uncovering the universality of self-replication in protein aggregation and its link to disease.

The paper investigates self-replication in protein aggregates, finding it common in both functional and pathological contexts, with disease-related aggregates replicating faster than biological ones.

resPAINT: Accelerating volumetric super-resolution localisation microscopy by active control of probe emission

The paper introduces resPAINT, a method that enhances volumetric super-resolution microscopy by actively controlling probe emission, achieving up to 50-fold faster localisation rates without sacrificing contrast.

AN ECONOMIC, SQUARE-SHAPED FLAT-FIELD ILLUMINATION MODULE FOR TIRF-BASED SUPER-RESOLUTION MICROSCOPY

The paper introduces a cost-effective flat-field illumination module for TIRF-based super-resolution microscopy to address uneven illumination issues.

Construction of a liquid-liquid phase separation system from the gel-sol transition of elongated protein microgels in a crowding agent

This study constructs a liquid-liquid phase separation system through a gel-sol transition of elongated protein microgels in a crowding agent, enabling tunable and monitorable models for drug release applications.

Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, while the Alzheimer's R47H mutant does the opposite

The paper shows wild-type sTREM2 inhibits Aβ aggregation and neurotoxicity, while the R47H variant promotes Aβ aggregation and neurotoxicity.

Amplification, not spreading limits rate of tau aggregate accumulation in Alzheimer's disease

The paper quantifies tau aggregate replication rates in Alzheimer's disease and finds local replication, not spreading, limits tau accumulation in neocortical regions.

Single-molecule light-sheet microscopy with local nanopipette delivery

This paper introduces ldSPIM, a method combining light sheet microscopy with nanopipette delivery to precisely deliver single molecules to cells and image their effects in real-time.

Robust T-cell signaling triggered on soft polydimethylsiloxane-supported lipid-bilayers

This study investigates T-cell signaling on soft lipid bilayers, finding early calcium signaling is not mechanosensitive but phosphatase exclusion occurs on soft substrates.

Direct digital sensing of protein biomarkers in solution

This paper introduces a single-molecule microfluidic platform for digital protein biomarker detection, demonstrating its ability to quantify protein concentrations and provide insights beyond traditional immunoassays.

Live-cell 3D single-molecule tracking reveals how NuRD modulates enhancer dynamics

This study uses live-cell 3D single-molecule tracking to show that the NuRD complex decompacts chromatin and alters enhancer dynamics by increasing their movement and reducing time in a fast-diffusing state.

A general in vitro assay to study enzymatic activities of the ubiquitin system

The paper introduces a FRET-based assay to measure ubiquitination kinetics in vitro, demonstrating its versatility and compatibility with various enzyme combinations.

ThX - A next-generation probe for the early detection of amyloid aggregates

This paper introduces ThX, a new fluorescent probe for detecting amyloid aggregates, showing improved brightness and binding affinity over ThT.

The costs of close contact: Visualizing the energy landscape of cell contacts at the nanoscale

This paper uses quantum dots to study how protein size affects diffusion and entry into cell contacts, revealing size-dependent exclusion and reduced diffusion in dense adhesion protein regions.

A cell-topography based mechanism for ligand discrimination by the T-cell receptor

This paper develops a model showing T-cell receptors discriminate ligands based on dwell-time in phosphatase-depleted contacts constrained by cell topography, predicting signaling potency without multi-step proof-reading.

N-terminal ubiquitination of amyloidogenic proteins triggers removal of their oligomers by the proteasome holoenzyme

The paper shows that N-terminal ubiquitination of amyloidogenic proteins by UBE2W alters their aggregation process, enabling proteasome targeting of soluble oligomers.

Filamentous aggregates are fragmented by the proteasome holoenzyme

This study shows the proteasome holoenzyme fragments amyloid fibrils into smaller, more toxic species, suggesting a novel degradation mechanism for filamentous aggregates.

The stoichiometric signature of Rhodopsin-family G protein coupled receptors

The paper analyzes 71 GPCRs from a single cell to show that Rhodopsin-family receptors predominantly exist as monomers rather than dimers.