P. Schanda

Deuteration of proteins boosted by cell lysates: high-resolution amide and Hα MAS NMR without re-protonation bottleneck

This paper demonstrates a method to overcome the re-protonation bottleneck in NMR by using deuterated cell lysates, enabling high-resolution amide and Hα MAS NMR with improved sensitivity.

Disulfide-bond-induced structural frustrationand dynamic disorder in a peroxiredoxin fromMAS NMR

This study investigates how disulfide bonds affect the dynamics of peroxiredoxin assemblies using NMR, finding structural frustration and dynamic disorder.

Aromatic ring flips in differently packedubiquitin protein crystals from MAS NMR andMD

The paper investigates how crystal packing affects aromatic ring flips in ubiquitin using NMR and MD simulations, finding that intramolecular factors dominate over packing effects.

Functional control of a 0.5 MDa TET aminopeptidase by a flexible loop revealed by MAS NMR

The paper uses MAS NMR and computational methods to show a flexible loop in TET2 aminopeptidase is critical for catalytic function and substrate processing.

Structural basis of client specificity in mitochondrial membrane-protein chaperones

The paper determines how two mitochondrial chaperones bind different membrane proteins by analyzing their structural interactions and identifies factors that influence client specificity.

Architecture and assembly dynamics of the essential mitochondrial TIM chaperone systems

The paper derives a structural model of the TIM9{middle dot}10{middle dot}12 complex using NMR, X-ray scattering, and MD simulations, revealing dynamic subunit exchange and conformational changes.

Mechanism of the allosteric activation of the ClpP protease machinery by substrates and active-site inhibitors

The paper investigates how bortezomib activates ClpP protease by mimicking a substrate and inducing allosteric conformational changes that enhance its interaction with ClpX.

Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex

The paper integrates NMR and cryo-EM to determine the atomic structure of a large enzyme complex, achieving high precision and accuracy beyond the limitations of each method individually.

Slow conformational exchange and overall rocking motion in ubiquitin protein crystals

This study investigates how crystal packing affects microsecond dynamics of ubiquitin using solid-state NMR and MD simulations, revealing altered thermodynamics and kinetics of conformational exchange.