T. Higashiyama

This paper develops a microfluidic device to detect miR399 in plant extracts for early diagnosis of phosphorus deficiency stress.

This study performs transcriptomic analysis of Arabidopsis thaliana funiculus in stk mutants to identify deregulated genes and processes involved in seed development.

This study identifies the role of NASP in de novo CENH3 deposition during Arabidopsis early embryogenesis and shows its species-specific interaction with CENH3.

The paper identifies a mechanism for seed size regulation through callose deposition/degradation at the phloem end of the ovule, mediated by β-1,3-glucanase genes.

This study quantifies the motility differences between sperm packets and asexual colonies of Pleodorina starrii, revealing distinct flagellar waveform patterns and motility characteristics.

This study identifies HDA19's role in downregulating STM in the CMM to enable ovule formation and transmitting tract differentiation in Arabidopsis.

The paper uses two-photon imaging to study pollen tube dynamics and signaling in one-to-one fertilization of ovules in angiosperms, revealing spatiotemporal regulation mechanisms.

This study shows F-actin is essential for polarized secretion of attractants in Arabidopsis synergid cells, affecting pollen tube guidance.

The paper identifies two plant cell division inhibitors through chemical screening in Arabidopsis zygotes and validates their effects in tobacco BY-2 cells and multiple plant species.

This study identifies IZUMO1 as a unilateral gamete fusogen, showing it can induce cell fusion independently of JUNO binding.

This study shows plasma membrane damage limits replicative lifespan in yeast and induces premature senescence in human fibroblasts through the ESCRT pathway and Ca2+-p53 axis.

This study shows that trehalose supplementation remodels the human gut microbiota, preventing simulated Clostridioides difficile infection in an in vitro model.

This study delivers CRISPR/Cas9 and fluorescent markers to pollen tubes using biolistic delivery, demonstrating successful gene editing and marker expression in male germ cells.

This study visualizes female gametophyte development in Arabidopsis and identifies when and how cell fates are determined during its formation.

This study identifies β-1,4-glucanases as facilitators of cell-cell adhesion in plant grafting, showing their role in promoting compatibility across species.

The paper develops a micrografting chip to simplify and improve the efficiency of micrografting in Arabidopsis for studying systemic signaling.

This study shows that KAKU4 controls vegetative nucleus shape, influencing its migration order over sperm cells in pollen tubes and affecting pollen competitiveness.

This study identifies two peptides, EPFL2 and EPFL9, and their receptors that coordinate ovule spacing, seed number, and fruit size in Arabidopsis thaliana through auxin feedback.