Eric J. Wagner

Dual kinase inhibition provides early clues to restoration of neuroimmune homeostasis in an AAV-haSyn model of Parkinsons disease

This study shows that dual inhibition of MLK3 and LRRK2 with URMC-099 reduces PD pathology and motor deficits in an AAV-haSyn rat model.

CDK12 and CDK13 suppress distinct intronic polyadenylation sites

This study shows CDK12 and CDK13 suppress distinct intronic polyadenylation sites, leading to premature termination and novel peptides in cancer.

HIV-1 Infection Regulates Gene Expression by Altering Alternative Polyadenylation Through CPSF6 and CPSF5 Delocalization

A UL26-PIAS1 complex antagonizes anti-viral gene expression during Human Cytomegalovirus infection

This study identifies the UL26-PIAS1 complex as a mechanism by which HCMV inhibits anti-viral gene expression and promotes cell-to-cell spread.

Regulation of the Drosophila transcriptome by Pumilio and CCR4-NOT deadenylase

This study identifies novel Pumilio target genes and reveals how Pumilio and CCR4-NOT deadenylase regulate mRNA stability and translation efficiency in Drosophila.

PolyAMiner-Bulk: A Machine Learning Based Bioinformatics Algorithm to Infer and Decode Alternative Polyadenylation Dynamics from bulk RNA-seq data

PolyAMiner-Bulk identifies and decodes alternative polyadenylation dynamics from bulk RNA-seq data, revealing novel APA changes in disease contexts and Alzheimer's Disease.

Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq

The paper performs genome-scale Perturb-seq to map genotype-phenotype relationships, uncovering new gene functions and stress-specific mitochondrial regulation.

RBFOX2 Is Critical For Maintaining Alternative Polyadenylation And Mitochondrial Health In Rat Myoblasts

This study identifies RBFOX2's role in maintaining alternative polyadenylation and mitochondrial health in rat myoblasts, linking it to heart disease pathology.

Inositol hexakisphosphate is a critical regulator of Integrator assembly and function

The paper identifies inositol hexakisphosphate (IP6) as a critical regulator of Integrator assembly and function in Drosophila and humans.

Genetic Basis of Alternative Polyadenylation is an Emerging Molecular Phenotype for Human Traits and Diseases

The paper identifies an atlas of 3QTLs associated with alternative polyadenylation across 46 tissues, showing they explain 16.1% of trait-associated variants and contribute to disease independently of gene expression.

Human Pumilio proteins directly bind the CCR4-NOT deadenylase complex to regulate the transcriptome

This study identifies how PUM1 and PUM2 regulate gene expression by interacting with the CCR4-NOT complex and DCP2 to repress mRNA decay.

Partial loss of CFIm25 causes aberrant alternative polyadenylation and learning deficits

The paper shows that partial loss of CFIm25, a protein regulated by NUDT21, causes learning deficits and molecular changes in neurons.

The Integrator complex terminates promoter-proximal transcription at protein-coding genes

The paper shows the Integrator complex destabilizes paused RNA polymerase II at promoters, leading to termination of non-productive transcription.

The Integrator complex cleaves nascent mRNAs to attenuate transcription

The paper identifies the Integrator complex as a regulator of transcription by cleaving nascent mRNAs, revealing its role in attenuating gene expression during copper stress.

Co-evolutionary analysis accurately predicts details of interactions between the Integrator complex subunits

The paper modifies Direct Coupling Analysis to predict interaction domains in large protein complexes, validated on known and unknown complexes.

Composition of the Survival Motor Neuron (SMN) complex in Drosophila melanogaster

The paper identifies CG2941 as a Gemin4 ortholog in Drosophila melanogaster and shows its essential role in organismal viability.

Integrator Subunit 4 is a ‘Symplekin-like’ Scaffold that Associates with INTS9/11 to Form the Integrator Cleavage Module

The paper identifies INTS4 as a conserved interaction partner of INTS9/11, forming a heterotrimeric complex critical for UsnRNA biogenesis.

Poly(A)-ClickSeq: click-chemistry for next-generation 3´-end sequencing without RNA enrichment or fragmentation

Poly(A)-ClickSeq enables high-quality RNA-seq libraries of poly(A) site junctions without RNA enrichment or fragmentation, revealing new poly(A) sites and APA regulation.