cryo-EM

The paper introduces a modification to cryo-FIB milling patterns by adding fillets to reduce stress concentration and lamella breakage, improving yield by 33%.

This paper introduces tilt-corrected bright-field scanning-TEM (tcBF-STEM) for cryo-EM, showing improved contrast and efficiency for thick samples, with a subnanometer resolution SPA map of a virus-like particle.

This paper reports the cryo-EM structure of human adenovirus 7 virus-like particles, revealing interactions critical for capsid maturation and stability.

The paper presents cryo-EM structures of the fungal ABC pump Cdr1, revealing its ATP-driven dynamics and drug-binding mechanism.

The paper identifies a novel structural motif in the HAdV-D10 capsid and reports overexpression of a key transcript, providing insights for vector development.

This paper shows PCMTD1, a cullin-RING ligase, recognizes L-isoaspartyl residues and targets damaged proteins for ubiquitylation and degradation.

The paper reports cryo-EM structures of avian IgA and compares them to mammalian IgA, revealing structural differences that may affect mucosal immune responses.

The paper investigates how the human ribosome modulates multidomain protein folding by delaying domain docking, contrasting with bacterial ribosomes.

The paper determines the in situ structures of respiratory supercomplexes and ATP synthase oligomers in bovine heart mitochondria using cryoEM, revealing new structural arrangements and their implications for mitochondrial function.

This study reveals a structural mechanism for thermosensing in TRPV3 channels, showing that extreme thermosensitivity arises from protein unfolding and dynamic changes rather than localized sensors.

This study determines the cryo-EM structure of mTORC1 on the lysosomal membrane, showing how growth factor and nutrient signals integrate through three-step recruitment and membrane engagement.

This study identifies how Plasmodium tubulin adaptations lead to distinct microtubule structures, mechanics, and drug susceptibility compared to mammalian microtubules.

This study presents cryo-EM structures of ChR024, revealing its color-tuning mechanism and transition from a proton pump to a light-gated channel.

This paper introduces a combined MSI and cryo-ET pipeline for reliable single-cell classification in cryo-ET samples, enabling post-acquisition cell identification and classification.

The paper identifies structural determinants of far-red absorption in Photosystem I of Arabidopsis by analyzing cryo-EM structures and quantum mechanics calculations.

This paper introduces a method to overcome preferred orientation in cryo-EM by using ultrasonic excitation during vitrification, improving data acquisition efficiency.

CryoPhold integrates AlphaFold, cryo-EM, and molecular simulations to predict protein dynamics and structural hotspots in human proteins like GlyT1 and BRAF mutants.

This study presents the cryo-EM structure of the R2C complex, revealing how CCDC103 interacts with RUVBL1-RUVBL2 to facilitate dynein motor assembly in PCD.

The paper identifies Rad51 interaction motifs in Rad54 and Hed1, and solves their CryoEM structures to explain their roles in regulating Rad51 activity during mitosis and meiosis.

The paper uses cryo-ET to study mitochondrial ATP synthase dynamics in Chlamydomonas reinhardtii, revealing conformational states and movement patterns of its components.

This study presents cryo-EM structures and functional analyses of TRPM8 activation by cold and menthol, revealing mechanisms of cold sensing and menthol specificity.

The paper introduces a method to separate atomic displacement and local resolution effects in crystallographic and cryo-EM maps by modeling Fourier ripples.

This paper presents a workflow for cryo-EM structure determination of small proteins using heterogeneous ab initio reconstruction, successfully solving structures of three small proteins with resolutions below 5 Å.

This paper introduces a genetically encoded FerriTag system for labeling target proteins in live cells for cryo-electron tomography, enabling precise localization with high contrast.

This study investigates the role of the linker domain in ABCB1/P-glycoprotein using simulations, cryo-EM, and computational methods, revealing its impact on protein structure and function.

The paper presents a cryo-EM structure of a Cyt c:PSI complex from Chlamydomonas reinhardtii, revealing key interactions involved in electron transfer.

The paper uses cryo-EM to study the NifL-NifA complex in Azotobacter vinelandii, revealing how NifL sequesters NifA to regulate nitrogen fixation and enabling engineered strains with enhanced nitrogen secretion.

This study uses in situ cryo-ET to show HPV remains intact during retrograde transport and lysosome residency, challenging existing models of viral disassembly and transport.

This paper introduces cryo-SXT as an intermediate technique to bridge the resolution gap in cryo-CLEM by providing 3D cellular context to cryo-ET datasets.

This paper introduces a method to validate 3D cryoEM reconstructions by separating scattering contributions into real and imaginary components to determine handedness and assess reconstruction quality.