cryo-EM

The paper develops a computational method to measure membrane thickness in cryo-electron tomograms, revealing systematic variations across organelles and supporting hydrophobic matching models.

The paper determines the cryo-EM structure of shutdown human non-muscle myosin 2A, revealing ionic interactions and explaining differences in shutdown mechanism compared to β-cardiac myosin.

The paper reports cryo-EM structures of the human 26S proteasome bound to K11/K48-branched ubiquitin chains, revealing a multivalent recognition mechanism involving new binding sites for K11 and alternating K11-K48 linkages.

This paper presents the cryo-EM structure of the Gloeobacter violaceus phycobilisome, revealing its unique bundle-shaped architecture and regulation by orange carotenoid proteins.

This study uses cryo-EM to analyze HCN1 channel variants, revealing structural differences in cAMP sensitivity and auto-inhibition mechanisms between HCN1 and HCN2 isoforms.

This study investigates the structural and dynamic organization of plasma membrane proteins in Candida glabrata using cryo-ET and live cell imaging, revealing how echinocandin drugs affect their distribution and function.

This study determines the structural basis of multitasking by the apicoplast DNA polymerase from Plasmodium falciparum using cryoEM, revealing how it balances replicative and lesion bypass synthesis.

The paper presents the cryo-EM structure of the MecA/ClpC/ClpP complex from Staphylococcus aureus, revealing how MecA facilitates substrate transfer and how ClpC/ClpP interactions are regulated by ATPase activity.

The paper presents a 3.3 Å cryo-EM map of Vibrio cholerae competence pilus and a full-length pseudoatomic model of the CPM, revealing unique structural features and mechanical properties.

This study identifies donor strand complementation and calcium ion coordination as key drivers of chaperone-free cannulae polymerization in archaea.

This paper introduces a method using Ni-NTA lipid monolayers to improve cryo-EM grid preparation, enabling high-resolution structure determination of proteins and the nucleosome.

This study determines the cryo-EM structure of the DosR-dependent transcriptional activation complex in Mtb and identifies its molecular mechanism for dormancy regulation.

This study reports the high-resolution cryo-EM structure of native human nucleosomes from HEK293 cells and provides mass spectrometry analysis of histone modifications.

This paper optimizes adhesion methods for cryo-electron tomography of vitreous sections, increasing throughput to match thin film methods and enabling high-resolution imaging of molecular complexes.

The paper reports a cryo-EM structure of far-red Photosystem I from Chroococcidiopsis thermalis, revealing all eight chlorophylls f and their functional roles in photochemistry.

This paper determines cryo-EM structures of an anti-MLC1 Fab in apo and peptide-bound states, revealing the structural basis of antigen recognition and demonstrating cryo-EM's capability for small Fab-antigen complexes.

This study identifies a hydration network in GPR174 that drives receptor activation and G protein selectivity through structural and functional analyses.

This study presents the cryo-EM structure of PLCε, revealing its N-terminal domains and their role in membrane engagement and activity.

ANTIDOTE improves cryoEM particle sorting by using metadata-driven neural networks, enhancing reconstruction quality and reducing manual curation efforts.

The paper presents cryo-EM structures of human mitochondrial Hsp60 chaperonin in different states to elucidate the structural basis of ATP-driven double-ring assembly.

This study uses cryo-EM to determine the near-atomic structure of the archaeal virus HFTV1, revealing insights into its assembly, infection mechanisms, and host-virus interactions.

The paper determines the cryo-EM structure of ADGRL4 coupled to Gq, showing weak Gq coupling and distinct interactions compared to other GPCRs, supporting its role in cancer.

This study determines the structure of KCNQ1+KCNE1 in closed and open states, revealing structural transitions and dual PIP2 binding that regulate channel gating and IKs function.

This paper develops a few-shot learning framework for cryo-EM micrograph classification using triplet loss embeddings, achieving competitive performance with minimal labeled examples.

This paper introduces Rigid-Fabs, disulfide-constrained antibody fragments that enable high-resolution cryo-EM structures of small proteins by overcoming size limitations.

This study shows that lipid concentration affects RyR1 open probability under cryo-EM conditions, with higher concentrations increasing open probability but lipid nanodiscs keeping RyR1 closed.

This study identifies how RhoA activates PLCε through a unique EF hand insertion, enhancing membrane interaction and cardiomyocyte survival during ischemia/reperfusion.

This study reveals how tetracyclines target the bacterial ribosome using cryo-EM structures, showing species-specific interactions and a new mechanism for antibiotic action.

The paper determines the structures of two actinoporins in a membrane environment using cryogenic electron microscopy, revealing their pore-forming mechanism through intermediate states.

This study presents a cryo-EM structure of H5N1 influenza polymerase bound to the cRNA promoter, revealing structural features essential for internal initiation of viral RNA synthesis.