Ion channel regulation and function

The paper investigates ionic mechanisms underlying bistability in spinal motoneurons using a computational model and mouse recordings, identifying key currents involved in plateau potentials and bistable firing.

This study investigates how missense mutations in the CTD of Nav1.5 affect gating properties, revealing distinct effects on inactivation and activation.

This paper designs Ca2+ channels with specific selectivity filter geometries using a bottom-up approach, showing higher Ca2+ conductance and structural accuracy.

This study identifies fentanyl's off-target blockade of EAG-class potassium channels in motoneurons as a mechanism underlying Wooden Chest Syndrome, independent of opioid receptor activation.

This study uses super-resolution imaging to reveal the dynamic nanoscale architecture of CaV1.3 channel clusters in cardiac myocytes, showing mobile channels within nanodomains rather than dense packing.

The paper identifies L-type calcium and SK-type potassium currents as essential for tonic firing in larval zebrafish Purkinje neurons, proposing a conserved mechanism for sustained high-frequency firing.

This study identifies molecular determinants of early-stage folding in Kv1.3 channels, revealing dynamic interfaces and domain interactions that influence tetramerization and conformation.

This study identifies intrinsic biophysical properties of gamma motoneurons in mice, revealing mechanisms underlying their low recruitment threshold and high firing rates.

This study determines the structure of KCNQ1+KCNE1 in closed and open states, revealing structural transitions and dual PIP2 binding that regulate channel gating and IKs function.

The paper identifies Mangostin as a BK channel activator that induces vasorelaxation by modulating key S6 domain residues.

This study uses automated and manual patch-clamp techniques to analyze Cav3.1 channel variants, identifying gain- and loss-of-function properties linked to neurological disorders.

This study shows that lipid concentration affects RyR1 open probability under cryo-EM conditions, with higher concentrations increasing open probability but lipid nanodiscs keeping RyR1 closed.

The paper uses atomistic simulations to study voltage activation of BK channels, revealing unique VSD movement and coupling mechanisms distinct from canonical Kv channels.

The paper uses molecular dynamics simulations to reveal the atomistic mechanism of cation permeation and voltage sensitivity in the CNGA1 ion channel.

The paper investigates the structural gating mechanisms of YnaI, showing it opens via radial relocation of sensor paddles and pore shortening, contrasting with MscS's tilt-based mechanism.

This study investigates how sevoflurane affects Nav1.3 sodium channels in developing neurons, showing voltage-dependent inhibition and altered inactivation properties.

IonBench evaluates optimization strategies for ion channel models, identifying an efficient approach that reduces computation time and improves reliability.

This study uses time-resolved tmFRET to analyze conformational changes and energetics in the CNBD of SthK, showing that transmembrane domains make the activating conformation more favorable.

This study investigates how lithium isotopes affect sodium channel activity in SH-SY5Y and iPSC-derived cortical neurons, finding no significant differences in SH-SY5Y cells but small differences in iPSC-derived neurons.

This paper reveals the atomistic mechanism of voltage gating in TREK K2P channels through molecular dynamics simulations and electrophysiology, showing how SF conformational flexibility and water influx regulate ion permeation.

The paper solves the cryo-EM structure of Arabidopsis GORK, revealing symmetry reduction driven by ANK dimerization, which regulates K+ channel activity in guard cells.

The paper identifies intermediate voltage-sensor conformations in hERG channels caused by LQTS-associated mutations using FLIM, tmFRET, and MD simulations.

This study investigates the functional role of the I-II linker in Nav1.5 channels, finding that a point mutation significantly affects channel gating while deletions have minimal impact.

This study examines how diclofenac and eugenol modulate Kv7 and TRPV1 ion channels, showing enhanced analgesic effects when combined in animal models.

This study investigates how KCNE1 and KCNE3 modulate KCNQ1 gating through PIP2-dependent mechanisms, revealing distinct roles in voltage sensitivity and GPCR regulation across cell types.

This study determines the structure of human BASIC with and without Ca2+ using cryo-EM and shows Ca2+-dependent conformational changes in the transmembrane domain and β-linkers, revealing how Ca2+ modulates ion flow through the channel.

This study investigates the distinct roles of NaV1.2 and NaV1.6 in neocortical pyramidal cell excitability using inhibitors and genetic modifications to selectively block each isoform.

The paper demonstrates a method to enhance single-channel transport detection by confining substrate diffusion in membrane tethers, enabling observation of individual Ca2+ transport events.

The paper traces the evolutionary changes in the ENaC delta-subunit in cetartiodactyls, suggesting it functions in sodium sensing and became obsolete in marine cetaceans.

This study uses cryo-EM to examine BK channel structures with locked voltage sensor states, revealing how voltage and calcium binding interact to control channel gating.