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Unavoidable Disasters: the Hsp70 Chaperone Can Only Prevent Aggregation Over a Limited Time Span

The paper develops a computational model to show that Hsp70 chaperones only delay, not prevent, protein aggregation, depending on client-specific kinetic and thermodynamic properties.

Learning from Hi-C reveals nuclear dynamical reorganization in budding yeast across the cell cycle

This paper uses Hi-C data to infer 3D genome structure and chromatin dynamics in budding yeast without prior assumptions about nuclear organization.

Formation of perinuclear actin structures is driven by mechanosensitivity of actin polymerization

The paper develops a computational model to explain how mechanical stimuli drive the formation of perinuclear actin structures through mechanosensitive actin polymerization.

Lateral Membrane Heterogeneity Shapes Antimicrobial Association and Convergent Mechanical Responses

This study shows lateral membrane heterogeneity influences antimicrobial association and leads to a common loss of membrane mechanical stability across different compounds.

Nucleobase-Specific Recognition of DNA by Protamine

This study shows that protamines, despite being intrinsically disordered, bind DNA with nucleobase-specific preferences, favoring GC over AT in the major groove and vice versa in the minor groove.

Bacterial-Mimetic Membranes Reveal Hopanoid-Associated Domain-Like Lateral Heterogeneity

This study shows bacterial-inspired membranes with hopanoids spontaneously form distinct lateral domains, revealing how lipid composition affects membrane properties and behavior.

One high-order ion-coupling mechanism generates multiple SLC1A stoichiometries

This study identifies a high-order ion-coupling mechanism in EAAT transporters by showing that dEAAT2 can acquire Na+, H+, and K+ dependence through mutations, mimicking human hEAAT2's complex ion coupling.

FLASH radiotherapy drives intestinal regeneration via macrophage-derived WNT4 signaling and attenuated T-cell inflammation

This study shows FLASH radiotherapy promotes intestinal regeneration by preserving stem cells, enhancing WNT4 signaling, and reducing T-cell inflammation in mice.

Expression-System Biases Compromise the Functional Assessment of KCNH2 Variants and Clinical Risk Assessment

This study shows that common dual-expression systems fail to balance WT and variant hERG expression, leading to inaccurate functional assessments of KCNH2 variants.

Mechanical Fidelity of Fixed-Cell Traction Force Microscopy

This study evaluates how fixation affects traction force microscopy measurements, finding that glutaraldehyde preserves live-cell-like metrics better than other protocols.

Dynamical Fingerprints of Rare-Event Transitions from Transition Corridors

The paper introduces a method to identify key players in rare conformational transitions of proteins by analyzing transition corridors and testing their impact through computational and mutational approaches.

Elucidation of Sequence Constraints for Protein Folding on the Ribosome

This study investigates how ribosome-mediated protein folding imposes sequence constraints by analyzing mutations in E. coli DHFR using deep mutational scanning and fluorogenic sensors.

Microenvironmental Fingerprint of Maturing Tau Condensates from Spectral and Lifetime Phasor Analysis of a Solvatochromic Dye

This paper develops a multimodal framework using spectral and lifetime phasor analysis to characterize microenvironmental changes in Tau condensates during maturation.

Dual-reporter assay for selective small molecule inhibition of expanded toxic huntingtin

The paper introduces a dual-reporter assay to screen small molecules for selective inhibition of expanded toxic huntingtin, identifying artifacts and validating compounds.

A Deformulating Multi-Attribute SEC Method for Simultaneous Quantification of Total RNA, Conjugated Antibody, and Free Antibody in Targeted Lipid Nanoparticles

This paper introduces an online deformulating SE-HPLC method to simultaneously quantify total RNA, conjugated antibody, and free antibody in targeted lipid nanoparticles.

Engineering Nanobody Conformational Switches

This paper engineers conformational switches using nanobodies that fold upon ligand binding, enabling customizable receptors and sensors for biomarkers and drugs.

Nanomechanical phenotyping uncovers a non-canonical smooth muscle-like hypercontractility program in lung squamous cell carcinoma myofibroblasts

The paper identifies a non-canonical smooth muscle-like hypercontractility program in LUSC myCAFs using nanomechanical phenotyping, linking it to tumor mechanics and adverse outcomes.

FACT frees Ubp10 from nucleosome sequestration after rapid H2B deubiquitination

The paper shows that FACT facilitates Ubp10 release from nucleosomes after H2B deubiquitination, revealing release as the rate-limiting step for Ubp10 turnover.

Frequency-Dependent Structural Responses of Amyloid-β Fibrils to Terahertz Pulses: A Nonequilibrium Molecular Dynamics Study

This study uses nonequilibrium molecular dynamics to show frequency-dependent structural changes in amyloid fibrils under terahertz pulses, identifying two electric-field thresholds for disruption.

Maximum Likelihood Localization Maps

This paper introduces a non-iterative method to create molecular probability maps from single-molecule localization data, preserving positional likelihood without coordinate-based rendering.