A. Shevchenko

Multi-species benchmark analysis for LC-MS/MS validation and performance evaluation in bottom-up proteomics

The paper introduces a benchmarking procedure and software for evaluating LC-MS/MS performance in bottom-up proteomics, enabling comparison of workflows and error detection.

Extracellular vesicles of Euryarchaeida: precursor to eukaryotic membrane trafficking

This study identifies RNA-containing extracellular vesicles in Haloferax volcanii, suggesting a potential RNA communication system and linking archaeal EV formation to eukaryotic membrane trafficking origins.

Absolute quantification of photoreceptor outer segment proteins

This study uses quantitative mass spectrometry to determine the absolute protein abundances in mouse rod outer segments, resolving discrepancies and showing high precision in molar ratios.

Activation of mTOR by release of extracellular cholesterol stores controls the transition from quiescence to growth in C. elegans

The paper shows that cholesterol release via SCL-12 and SCL-13 depletion activates mTOR, enabling C. elegans to transition from dauer quiescence to growth.

Distinct glycerophospholipids potentiate Gsα-activated adenylyl cyclase activity

The paper identifies specific glycerophospholipids that enhance Gsα-activated adenylyl cyclase activity, suggesting an intracellular regulatory role.

FastCAT accelerates absolute quantification of proteins by using multiple short non-purified chimeric standards

FastCAT improves absolute protein quantification by using chimeric standards, achieving accuracy and efficiency in quantifying neurological markers in human cerebrospinal fluid.

Median based absolute quantification of proteins using Fully Unlabelled Generic Internal Standard

This paper introduces a method for absolute protein quantification using a fully unlabelled generic internal standard, showing improved performance over existing methods.

Shotgun lipidomics and mass spectrometry imaging unveil diversity and dynamics in lipid composition in Gammarus fossarum

The paper uses shotgun lipidomics and mass spectrometry imaging to study lipid composition and spatial distribution in Gammarus fossarum, revealing dynamic lipid changes and sulfate-based lipids in oocytes.

A non-canonical lipid droplet metabolism regulates the conversion of alpha-Synuclein to proteolytic resistant forms in neurons of a Drosophila model of Parkinson disease

This study shows that lipid droplet proteins interact with alpha-synuclein in Drosophila neurons, promoting its resistance to proteolysis and aggregation, suggesting a role for non-canonical lipid metabolism in Parkinson's disease.

Recapitulating evolutionary divergence in a single regulatory element causes expression changes of the lens gene Tdrd7

The paper investigates how mutations in a regulatory element upstream of Tdrd7 affect lens development by replacing the mouse sequence with the blind mole rat sequence in mice.

An insulin, AMPK, and steroid hormone-mediated metabolic switch regulates the transition between growth and diapause in C. elegans

The paper shows that insulin, AMPK, and steroid hormone pathways regulate metabolic shifts during growth and diapause in C. elegans by altering enzyme ratios in central metabolic pathways.

Sterols as dietary markers for Drosophila melanogaster

The paper identifies sterol ratios as a marker for dietary shifts in Drosophila melanogaster during cold acclimation, offering a simpler alternative to full lipidome analysis.

Addressing lipid structural diversity in signalling: Photochemical probes for live-cell lipid biochemistry

This study develops photochemical probes to manipulate diacylglycerol species in live cells, revealing structural diversity impacts on protein binding and signaling.

Fine Endmesolithic Fish Caviar Meal Discovered by Proteomics of Foodcrusts

The paper uses proteomics to analyze foodcrusts from Mesolithic-Neolithic ceramics, identifying fine carp roe meal and revealing a prehistoric culinary recipe.

Body size-dependent energy storage causes Kleiber’s law scaling of the metabolic rate in planarians

This study shows Kleiber’s law in planarians arises from size-dependent energy storage, not cellular metabolic rate changes.

MS Western, a method of multiplexed absolute protein quantification is a practical alternative to western blotting

MS Western provides a method for absolute protein quantification without antibodies or labels, outperforming Western blotting in specificity and sensitivity.