A. Sinz

Twisting Urea- to Imide-Based Mass Spectrometry-Cleavable Cross-Linkers Enables Affinity Tagging

This paper introduces DSSI, a new class of MS-cleavable cross-linkers with affinity tags, and validates its use in structural biology.

Evidence that the Isc Iron-Sulfur Cluster Biogenesis Machinery Delivers Iron for -Cofactor Biosynthesis in Escherichia coli

The paper identifies the Isc machinery as the source of iron for the Fe(CN)2CO group in [NiFe]-hydrogenases in E. coli, while the Suf machinery can deliver the [4Fe-4S] cluster to HypD.

Structural Assessment of the Full-Length Wild-Type Tumor Suppressor Protein p53 by Mass Spectrometry-Guided Computational Modeling

This study uses mass spectrometry and computational modeling to assess the structure of full-length wild-type p53, revealing compaction of the C-terminal region and supporting a model of DNA binding.

Structural surfaceomics reveals an AML-specific conformation of Integrin-β2 as a CAR-T therapy target

The paper identifies an AML-specific conformation of Integrin-β2 using structural surfaceomics and demonstrates its targeting by CAR-T cells.

Delineating organizational principles of the endogenous L-A virus by cryo-EM and computational analysis of native cell extracts

The paper uses cryo-EM and computational analysis to study the structure and organization of the yeast L-A virus in native cell extracts, revealing capsid architecture and viral community dynamics.

First direct observation of 'elongated' conformational states in α-synuclein upon liquid-liquid phase separation

This study observes elongated conformational states of α-synuclein during liquid-liquid phase separation using XL-MS and COMPASS, providing insights into early stages of Parkinson's Disease.

RUNX1 isoform disequilibrium in the development of trisomy 21 associated myeloid leukemia

This study identifies RUNX1 isoform imbalance as a key driver of trisomy 21-associated myeloid leukemia and shows that restoring this balance can reverse leukemogenesis.

The megakaryocytic transcription factor ARID3A suppresses leukemia pathogenesis

This paper identifies ARID3A repression by miR-125b as a key driver of megakaryoblastic leukemia, showing that restoring ARID3A expression can reverse differentiation arrest in AMKL.

Cross-linking/Mass Spectrometry Combined with Ion Mobility on a timsTOF Pro Instrument for Structural Proteomics

This paper demonstrates the use of XL-MS combined with ion mobility on a timsTOF Pro instrument to study protein conformations and interactions, identifying numerous cross-links and interactions in different protein systems.

High-throughput characterization of photocrosslinker-bearing ion channel variants to map residues critical for function and pharmacology

The paper develops a high-throughput method to study functional and pharmacological properties of ncAA-containing hASIC1a variants using automated patch-clamp.

First 3D-Structural Data of Full-length Rod-Outer-Segment Guanylyl Cyclase 1 in Bovine Retina by Cross-linking/Mass Spectrometry

The paper presents the first 3D-structural analysis of active, full-length ROS-GC1 in bovine retina using XL-MS and computational modeling, revealing a novel domain organization and dimeric architecture.

IGF2BP1 is a targetable SRC/MAPK-dependent driver of invasive growth in ovarian cancer

This study identifies IGF2BP1 as a driver of invasive growth in ovarian cancer through SRC/MAPK signaling, showing that combinatorial SRC/MEK inhibition is effective in treating mesenchymal-like HG-SOC.

Structural Analysis of 70S Ribosomes by Cross-Linking/Mass Spectrometry Reveals Conformational Plasticity

This study uses XL-MS to analyze E. coli ribosome structures, revealing conformational plasticity and remodeling 2,057 residues with high accuracy.

Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients

The paper develops an MS-based method to detect SARS-CoV-2 proteins in gargle samples from COVID-19 patients using acetone precipitation and targeted MS analysis.

Flexibility of intrinsically disordered degrons in AUX/IAA proteins reinforces auxin co-receptor assemblies

The paper investigates how flexible intrinsically disordered regions in AUX/IAA proteins modulate auxin receptor assemblies by positioning AUX/IAAs on TIR1 for ubiquitylation.

Reconstitution of mammalian Cleavage Factor II involved in 3’ processing of mRNA precursors

This study reconstitutes mammalian Cleavage Factor II as a heterodimer of hPcf11 and hClp1, showing its activity in RNA cleavage and RNA binding preferences.

Translational Repression Of The Drosophila nanos mRNA Involves The RNA Helicase Belle And RNA Coating By Me31B And Trailer hitch

The paper identifies Belle as a novel component of the SRE-dependent repressor complex that contributes to translational repression of nanos mRNA in Drosophila.