C. M. Capitini

Label free metabolic imaging to enhance the efficacy of Chimeric Antigen Receptor T cell therapy

Autofluorescence imaging informs manufacturing conditions that enhance yield and metabolic fitness of CAR T cells for neuroblastoma.

Virus-free CRISPR knock-in of a chimeric antigen receptor into KLRC1 generates potent GD2-specific natural killer cells

This study uses CRISPR to disrupt an inhibitory receptor in NK cells and insert a GD2-targeting CAR, creating potent NK cells against GD2+ melanoma.

Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence

This study shows that metabolic priming during CAR T cell manufacturing reduces glycolysis and enhances memory phenotypes, improving in vivo persistence against GD2+ tumors.

CD155 blockade enhances allogeneic natural killer cell-mediated antitumor response against osteosarcoma

This study shows CD155 blockade with allogeneic NK cell infusion improves survival against osteosarcoma in mice, with benefits mediated through DNAM-1.

Combination fedratinib and venetoclax has activity against human B-ALL with high FLT3 expression

This study shows that combining fedratinib and venetoclax effectively kills B-ALL cells with high FLT3 expression, both in vitro and in vivo.

Autofluorescence lifetime imaging classifies human lymphocyte activation and subtype

This paper uses optical metabolic imaging to classify human lymphocyte activation and subtype with high accuracy using autofluorescence lifetime parameters.

Inflammatory CD4/CD8 double positive human T cells arise from reactive CD8 T cells and are sufficient to mediate GVHD pathology

This study identifies CD4+/CD8+ double positive T cells as mediators of xenogeneic GVHD but with limited GVL activity.

Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression

This study produces and tests virus-free CRISPR-CAR T cells that effectively regress solid tumors in vivo.

MHC-matched allogeneic bone marrow transplant fails to eliminate SHIV-infected cells from ART-suppressed Mauritian cynomolgus macaques

This study shows that MHC-matched allogeneic bone marrow transplants in ART-suppressed SHIV-infected macaques do not fully eliminate viral reservoirs, with persistent SHIV DNA in tissues and rebound after ART interruption.

CXCR4 allows T cell acute lymphoblastic leukemia to escape from JAK1/2 and BCL2 inhibition through CNS infiltration

This study shows that combining JAK1/2 and BCL2 inhibitors in T-ALL leads to CNS infiltration, which is reduced by adding a CXCR4 inhibitor.