K. Saha

Label free metabolic imaging to enhance the efficacy of Chimeric Antigen Receptor T cell therapy

Autofluorescence imaging informs manufacturing conditions that enhance yield and metabolic fitness of CAR T cells for neuroblastoma.

Virus-free CRISPR knock-in of a chimeric antigen receptor into KLRC1 generates potent GD2-specific natural killer cells

This study uses CRISPR to disrupt an inhibitory receptor in NK cells and insert a GD2-targeting CAR, creating potent NK cells against GD2+ melanoma.

Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence

This study shows that metabolic priming during CAR T cell manufacturing reduces glycolysis and enhances memory phenotypes, improving in vivo persistence against GD2+ tumors.

Wearable Disposable Electrotherapy

This paper introduces a disposable electrotherapy platform that eliminates electronic components and uses scalable manufacturing, enabling single-use applications for various medical and therapeutic purposes.

Striatonigral distribution of a fluorescent reporter following intracerebral delivery of genome editors

The paper shows that genome editing in the striatum leads to fluorescent reporter expression in the substantia nigra pars reticulata via anterograde axonal transport.

Autophagy Increases Occludin Levels to Enhance Intestinal Paracellular Tight Junction Barrier.

This study shows autophagy increases occludin levels, enhancing intestinal tight junction barrier function through ERK1/2 MAPK signaling.

Label-free imaging to track reprogramming of human somatic cells

This study uses label-free live-cell imaging and machine learning to track reprogramming of human somatic cells and identify high-quality iPSCs with ~95% accuracy.

Cooperative engagement and subsequent selective displacement of SR proteins define the pre-mRNA 3D structural scaffold for early spliceosome assembly

The paper identifies a mechanism where SR proteins cooperatively bind pre-mRNA to form a scaffold, which is then selectively displaced by excess U1 snRNP to facilitate spliceosome assembly.

Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression

This study produces and tests virus-free CRISPR-CAR T cells that effectively regress solid tumors in vivo.

Discovery of a pre-mRNA structural scaffold as a contributor to the mammalian splicing code

The paper investigates how a three-dimensional pre-mRNA structural scaffold influences spliceosomal component recruitment beyond traditional splice signals.

Nano-Emulsion Adjuvant Enhances Cross-presentation by Lipid Body Formation Independent of Glycolysis

This study shows that the nano-emulsion adjuvant Adjuplex enhances cross-presentation in dendritic cells without requiring glycolysis, relying instead on lipid body formation and ROS.

Structural disruption of exonic stem-loops immediately upstream of the intron regulates mammalian splicing

The paper shows that exonic stem-loops upstream of introns regulate splicing by modulating splicing factor accessibility.

Label-free Method for Classification of T cell Activation

This paper develops a label-free method using NAD(P)H and FAD autofluorescence imaging and machine learning to classify T cell activation states and subtypes with high accuracy.