L. Shi

BRD8 guards the pluripotent state by sensing and maintaining histone acetylation

This study shows BRD8 maintains pluripotency by sensing and maintaining histone acetylation, preventing the conversion of primed EpiSCs to naive ESCs.

Rapid High-Throughput Isolation and purification of Chicken Myoblasts Based on Deterministic Lateral Displacement Microfluidic Chips

This study develops a microfluidic platform for isolating chicken myoblasts using deterministic lateral displacement, achieving 80% recovery and 99% purity.

A subtle modification of modafinil-based DAT inhibitors changes conformational preference

The paper investigates how a subtle structural change in modafinil analogs affects their binding to the dopamine transporter, revealing conformational differences between sulfoxide and sulfide substitutions.

Engineering customized viral receptors for various coronaviruses

This paper develops a method to engineer customized viral receptors (CVRs) for various coronaviruses, enabling infection models for viruses lacking known receptors.

Virus-free CRISPR knock-in of a chimeric antigen receptor into KLRC1 generates potent GD2-specific natural killer cells

This study uses CRISPR to disrupt an inhibitory receptor in NK cells and insert a GD2-targeting CAR, creating potent NK cells against GD2+ melanoma.

Sarbecovirus RBD indels and specific residues dictating ACE2 multi-species adaptiveness

This study identifies RBM indel types in sarbecoviruses and their impact on ACE2 receptor usage across species, revealing how specific residues and structural features influence tropism and adaptiveness.

Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging

The paper introduces far-red chemigenetic kinase activity biosensors enabling five-color multiplexing, 4D imaging, and super-resolution via STED microscopy.

Linkers in bitopic agonists shape bias profile among transducers for the dopamine D2 and D3 receptors

The paper analyzes bitopic ligands for dopamine D2 and D3 receptors, showing that linker structure and stereochemistry significantly influence transducer bias profiles.

Unraveling activation-related rearrangements and intrinsic divergence from ligand-induced conformational changes of the dopamine D3 and D2 receptors

This study identifies activation-related conformational changes and intrinsic divergence in dopamine D2 and D3 receptors by analyzing structures and simulations, revealing ligand-induced conformational changes obscure key differences.

The in vitro functional profiles of fentanyl and nitazene analogs at the μ-opioid receptor - high efficacy is dangerous regardless of signaling bias

This study evaluates the in vitro functional profiles of fentanyl and nitazene analogs at the μ-opioid receptor, finding that high intrinsic efficacy in Gi signaling correlates with increased risk of intoxication, regardless of signaling bias.

Herpes zoster mRNA vaccine induces superior vaccine immunity over licensed vaccine in mice and rhesus macaques

This study compares a novel VZV mRNA vaccine (ZOSAL) with Shingrix in mice and rhesus macaques, showing ZOSAL induces stronger T cell immunity and safety.

The molecular mechanism of positive allosteric modulation at the dopamine D1 receptor

This study investigates the molecular mechanism of LY3154207's positive allosteric modulation at the dopamine D1 receptor using cryo-EM structures and molecular dynamics simulations.

Patient-derived organoids assist personalized treatment for a patient with multiple metastatic neuroendocrine carcinoma of the cervix

The paper uses patient-derived organoids to model metastatic neuroendocrine carcinoma of the cervix and guide personalized treatment based on drug screening.

Divergent iron-regulatory states contribute to heterogeneity in breast cancer aggressiveness

The paper identifies divergent iron-regulatory states in triple-negative breast cancer cells that correlate with invasive and non-invasive phenotypes and predict patient outcomes.

De novo design of highly selective miniprotein inhibitors of integrins αvβ6 and αvβ8

This paper designs highly selective miniprotein inhibitors for integrins αvβ6 and αvβ8 with picomolar affinity and therapeutic potential in a mouse fibrosis model.

A subtle structural modification of a synthetic cannabinoid receptor agonist drastically increases its efficacy at the CB1 receptor

This study identifies a structural modification in a synthetic cannabinoid receptor agonist that significantly increases its efficacy at the CB1 receptor, with implications for monitoring illicit drug risks.

CD155 blockade enhances allogeneic natural killer cell-mediated antitumor response against osteosarcoma

This study shows CD155 blockade with allogeneic NK cell infusion improves survival against osteosarcoma in mice, with benefits mediated through DNAM-1.

Combination fedratinib and venetoclax has activity against human B-ALL with high FLT3 expression

This study shows that combining fedratinib and venetoclax effectively kills B-ALL cells with high FLT3 expression, both in vitro and in vivo.

Protein interactome homeostasis through an N-recognin E3 ligase is a vulnerability in aneuploid cancer

The paper identifies UBR4 as a critical mediator of protein interactome homeostasis in aneuploid cancers, showing its inactivation causes proteotoxic stress and convergence of copy number and protein levels.

Integrated Drivers of Basal and Acute Immunity in Diverse Human Populations

The paper examines factors influencing immunity across diverse populations, finding ancestry and age significantly affect immune responses and disease severity.

A cataract-causing Y204X mutation of CRYβB1 promotes C-terminal degradation and higher-order oligomerization

This study identifies a cataract-causing mutation in CRYβB1 that leads to C-terminal truncation, increased proteolysis, and higher-order oligomerization, contributing to lens opacity.

Imaging Architecture of Granulomas Induced by Mycobacterium tuberculosis Infections with Single-Molecule FISH

The paper develops a method to image mRNA and protein markers in tuberculosis granulomas using smFISH derivatives, enabling classification of immune cell subtypes and their spatial distribution.

Reprogramming Intrahepatic Cholangiocarcinoma Immune Microenvironment by Chemotherapy and CTLA-4 Blockade Enhances Anti-PD1 Therapy

This paper shows that combining chemotherapy with CTLA-4 blockade reprograms the immune microenvironment of intrahepatic cholangiocarcinoma, enhancing the effectiveness of anti-PD1 therapy.

A cinnamyl alcohol dehydrogenase (CAD) like enzyme leads to a branch in the shikonin biosynthetic pathway in Arnebia euchroma

This study identifies AeHGO, a CAD-like enzyme, as the branch point enzyme in the shikonin biosynthetic pathway of A. euchroma, showing it catalyzes reversible oxidation/reduction of key intermediates.

Modulating the epigenetic state promotes the reprogramming of transformed cells to pluripotency in a line-specific manner

This study identifies line-specific barriers to reprogramming transformed cells and shows that MEK inhibition can overcome some of these barriers by modulating the epigenetic state.

A therapeutic hepatitis B mRNA vaccine with strong immunogenicity and persistent virological suppression

This paper develops an mRNA vaccine for chronic hepatitis B that shows strong immunogenicity and persistent virological suppression in mouse models.

Identification and Characterization of ML321: a Novel and Highly Selective D2 Dopamine Receptor Antagonist with Efficacy in Animal Models that Predict Atypical Antipsychotic Activity

This paper develops ML321, a highly selective D2 dopamine receptor antagonist, showing efficacy in animal models predictive of atypical antipsychotic activity with fewer side effects.

Crystal ribcage: a platform for probing real-time lung function at cellular resolution in health and disease

This paper introduces a transparent ribcage for real-time, cellular-resolution imaging of lung function in health and disease, revealing insights into tumor progression, immune responses, and disease remodeling.

FGFR1 governs iron metabolism via regulating post-translational modification of IRP2 in prostate cancer cells

This study shows FGFR1 promotes prostate cancer by enhancing iron metabolism through regulation of IRP2 post-translational modification and TFR1 expression.

Optical Metabolic Imaging Uncovers Sex- and Diet-dependent Lipid Changes in Aging Drosophila Brain

This study uses optical metabolic imaging to show diet and sex influence lipid metabolism in aging Drosophila brains, revealing IIS pathway involvement.