Author

C. Siebold

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 6 Biochemistry· 3 Cell Biology· 1

Signature Assays, Organisms & Methods

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Research Focus:protein folding & dynamics· 6 Approach:experimental assay· 4 Approach:statistical model· 4 Assay:structural biology· 4 Approach:deep learning· 3 Organism:human· 3 Approach:mechanistic / dynamical model· 3 Research Focus:cell signaling & trafficking· 2

Frequent Co-Authors

C. E. Coupland· 4 shared T. B. Ansell· 4 shared M. S. P. Sansom· 3 shared M. Sansom· 3 shared R. A. Corey· 3 shared R. Rohatgi· 3 shared A. I. Magee· 2 shared E. W. Tate· 2 shared

Activity by Year

2018-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 31 mutual co-author links

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4 shared papersBiochemistry

C. E. Coupland

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Author profile →

10 papers

Recent·Most cited

Mapping Structural and Dynamic Divergence Across the MBOAT family

T. B. Ansell, M. Healy, C. E. Coupland, M. S. P. Sansom, C. Siebold

This study uses molecular dynamics and bioinformatics to analyze structural and dynamic differences across MBOAT family members, identifying key features relevant to inhibitor design.

bioRxiv · Dec 18, 2023Biochemistry

The Energetics and Ion Coupling of Cholesterol Transport Through Patched1

B. Ansell, R. A. Corey, L. V. Viti, M. Kinnebrew, R. Rohatgi, C. Siebold, M. Sansom

The paper uses molecular dynamics simulations and biochemical assays to study cholesterol transport through PTCH1, finding energetic barriers and ion-coupling mechanisms for cholesterol export.

bioRxiv · Feb 14, 2023Biophysics

LipIDens: Simulation assisted interpretation of lipid densities in cryo-EM structures of membrane proteins.

T. B. Ansell, W. Song, C. E. Coupland, L. Carrique, R. A. Corey, A. L. Duncan, C. K. Cassidy, M. M. G. Guerts, T. Rasmussen, A. B. Ward, C. Siebold, P. J. Stansfeld, M. Sansom

LipIDens uses MD simulations to interpret lipid-like densities in cryo-EM maps of membrane proteins, revealing protein-lipid interactions.

bioRxiv · Jun 30, 2022Biophysics

Structure and function of the ROR2 cysteine-rich domain in vertebrate noncanonical WNT5A signaling

S. C. Griffiths, J. Tan, A. Wagner, L. L. Blazer, J. J. Adams, S. Srinivasan, S. Moghisaei, S. S. Sidhu, C. Siebold, H.-Y. H. Ho

This study determines the structure of ROR2's CRD and Kr domains, revealing a novel mechanism for WNT5A signaling and identifying the CRD's role in ROR2 function and secretion.

bioRxiv · Jul 26, 2021Biochemistry

Structure and Mechanism of Hedgehog Acyl Transferase

C. E. Coupland, S. A. Andrei, T. B. Ansell, L. Carrique, P. Kumar, L. Sefer, R. A. Schwab, E. F. X. Byrne, E. Pardon, J. Steyaert, A. I. Magee, M. S. P. Sansom, E. W. Tate, C. Siebold

The paper presents the cryo-EM structure of HHAT, revealing its mechanism for transferring palmitate to SHH and identifying a heme group essential for its function.

bioRxiv · Jul 8, 2021 · PreprintBiophysics

Relative Affinities of Protein-Cholesterol Interactions from Equilibrium Molecular Dynamics Simulations

T. B. Ansell, L. Curran, M. R. Horrell, T. Pipatpolkai, S. C. Letham, W. Song, C. Siebold, P. J. Stansfeld, M. S. P. Sansom, R. A. Corey

This study develops a method to determine protein-lipid binding affinities from MD simulations and validates it against experimental and computational methods.

bioRxiv · Jun 2, 2021Biophysics

Photochemical probe identification of the small-molecule binding site in a mammalian membrane-bound O-acyltransferase

T. Lanyon-Hogg, M. Ritzefeld, L. Zhang, B. Pogranyi, M. Mondal, C. D. Johnston, C. E. Coupland, L. Sefer, S. A. Andrei, J. Newington, A. I. Magee, C. Siebold, E. W. Tate

This study develops a photochemical probe to identify the small-molecule binding site in HHAT, leading to the discovery of a potent inhibitor and insights into MBOAT inhibition mechanisms.

bioRxiv · Oct 16, 2020 · PreprintBiochemistry

Sphingomyelin suppresses Hedgehog signaling by restricting cholesterol accessibility at the ciliary membrane

M. Kinnebrew, E. J. Iverson, B. Patel, G. V. Pusapati, J. H. Kong, K. A. Johnson, G. Luchetti, D. Covey, C. Siebold, A. Radhakrishnan, R. Rohatgi

This study identifies sphingomyelin's role in restricting cholesterol accessibility at the ciliary membrane, thereby suppressing Hedgehog signaling.

bioRxiv · Jul 16, 2019 · PreprintCell Biology

Robin Hood: non-fitting, non-smoothing image detrending for bleaching correction

R. Nolan, M. Iliopoulou, C. Siebold, E. Y. Jones, S. Padilla-Parra

The paper introduces Robin Hood, an automatic detrending algorithm for fluorescence microscopy that performs well at low intensities, and evaluates it using simulated and low photon budget live cell images.

bioRxiv · Jun 14, 2019 · PreprintBiophysics

Cholesterol interaction sites on the transmembrane domain of the hedgehog signal transducer and Class F G protein-coupled receptor Smoothened

G. Hedger, H. Koldsoe, M. Chavent, C. Siebold, R. Rohatgi, M. Sansom

The paper identifies cholesterol interaction sites on the transmembrane domain of the GPCR Smoothened using molecular dynamics simulations and free energy calculations.

bioRxiv · Aug 2, 2018Biophysics