This paper introduces a drug delivery system using ultrasound to trigger release from hydrogels containing gas vesicles, demonstrating targeted delivery in a rat colitis model.

bioRxiv · May 10, 2024 · PreprintBioengineering

Ultrasonic reporters of calcium for deep tissue imaging of cellular signals

Z. Jin, A. Lakshmanan, R. Zhang, T. A. Tran, C. Rabut, P. Dutka, M. Duan, R. C. Hurt, D. Malounda, Y. Yao, M. G. Shapiro

This paper introduces a genetically encoded ultrasonic calcium reporter (URoC) for noninvasive in vivo imaging of calcium signaling in mammals.

bioRxiv · Nov 9, 2023 · PreprintBioengineering

Geometric effects in gas vesicle buckling under ultrasound

H. Salahshoor, Y. Yao, P. Dutka, N. N. Nystrom, Z. Jin, E. Min, D. Malounda, G. J. Jensen, M. G. Shapiro

This study investigates how the geometry of gas vesicles affects their buckling behavior under ultrasound, showing diameter has an inverse cubic effect on buckling pressure while length has minimal impact.

bioRxiv · Jun 27, 2022Biophysics

Magneto-acoustic protein nanostructures for non-invasive imaging of tissue mechanics in vivo

W.-S. Kim, S. Min, S. K. Kim, S. Kang, H. Davis, A. Bar-Zion, D. Malounda, Y. H. Kim, S. An, J.-H. Lee, S. H. Bae, J. G. Lee, M. Kwak, S.-W. Cho, M. G. Shapiro, J. Cheon

This paper introduces magneto-gas vesicles (MGVs) for non-invasive in vivo imaging of tissue mechanics, showing improved sensitivity and resolution compared to existing methods.

bioRxiv · May 26, 2022Biochemistry

Ultrafast amplitude modulation for molecular and hemodynamic ultrasound imaging

C. Rabut, D. Wu, B. Ling, Z. Jin, D. Malounda, M. G. Shapiro

This paper introduces an ultrafast nonlinear ultrasound imaging technique that improves sensitivity, field of view, and frame rate for molecular and hemodynamic imaging.

bioRxiv · May 18, 2021Bioengineering

Measuring gas vesicle dimensions by electron microscopy

P. Dutka, D. Malounda, L. A. Metskas, S. Chen, R. C. Hurt, G. J. Lu, G. J. Jensen, M. G. Shapiro

The paper explains discrepancies in gas vesicle diameter measurements and provides accurate methods using electron microscopy.

bioRxiv · Jan 22, 2021Biochemistry

Genetically encoded nanostructures enable acoustic manipulation of engineered cells

D. Wu, D. Baresch, C. Cook, D. Malounda, D. Maresca, M. P. Abundo, D. R. Mittelstein, M. G. Shapiro

This paper demonstrates that genetically encoded gas vesicles can be manipulated by ultrasound to control engineered cells.

bioRxiv · Jul 6, 2019 · PreprintBioengineering

Acoustically Detonated Biomolecules for Genetically Encodable Inertial Cavitation

A. Bar-Zion, A. Nourmahnad, D. Mittelstein, S. Yoo, D. Malounda, M. Abedi, A. Lee-Gosselin, D. Maresca, M. G. Shapiro

This paper demonstrates the use of gas vesicles engineered to produce mechanical effects via ultrasound, enabling targeted cell lysis and therapeutic payload release in vitro, in cellulo, and in vivo.

bioRxiv · Apr 29, 2019 · PreprintBioengineering

Biomolecular Contrast Agents for Optical Coherence Tomography

G. J. Lu, L.-d. Chou, D. Malounda, A. K. Patel, D. S. Welsbie, D. L. Chao, T. Ramalingam, M. G. Shapiro

This paper introduces gas vesicles as genetically encodable OCT contrast agents, enabling molecular and cellular imaging with OCT through differential refractive index and dynamic signal modulation.

bioRxiv · Mar 31, 2019Bioengineering