E. Marklund

High-Throughput DNA melt measurements enable improved models of DNA folding thermodynamics

This paper introduces a high-throughput method to measure DNA melting and develops models to improve DNA folding thermodynamics predictions.

A 2-Hydroxybutyrate-mediated feedback loop regulates muscular fatigue

This study shows that 2-hydroxybutyrate (2HB) produced during exercise inhibits branched-chain aminotransferases, leading to metabolic changes that enhance oxidative capacity.

Short tandem repeats bind transcription factors to tune eukaryotic gene expression

This study shows STRs modulate TF-DNA interactions by altering binding affinities and on rates, impacting gene expression through increased TF density near target sites.

Sequence specificity in DNA binding is determined by association rather than dissociation

The paper derives an equation linking association and dissociation rates in DNA-protein binding and tests it using LacI and dCas9, showing sequence specificity is determined by recognition efficiency, not dissociation rates.

Mechanism of DNA surface exploration and operator bypassing during target search

This study uses SMCT-FCS to show LacI slides on DNA, bypassing target sequences by hopping out of the DNA groove, revealing a speed-accuracy trade-off in target search.

Direct observation of rotation-coupled protein diffusion along DNA on the microsecond timescale

This study measures the rotation-coupled diffusion of LacI along DNA, finding it moves ~50 base pairs per revolution, supporting a facilitated diffusion model.