F. Pampaloni

mTOR controls growth and internal architecture of human breast cancer spheroids

This study investigates how mTOR signaling, through mTORC1 and mTORC2, influences the growth and internal architecture of human breast cancer spheroids.

Laser patterning bioprinting using a light sheet-based system equipped with light sheet imaging produces long-term viable skin constructs

This paper introduces a light sheet-based bioprinter with live imaging that produces long-term viable skin constructs with high resolution.

Combining organotypic tissue culture with multi-color fluorescence light-sheet microscopy (OTCxLSFM) - a novel tool to study glioma invasion/migration

This study introduces OTCxLSFM, a method combining organotypic tissue culture and light-sheet microscopy to analyze glioma invasion, showing that arsenic trioxide and CD9 depletion block tumor infiltration.

Species-specific LUBAC-mediated M1 ubiquitination regulates necroptosis by segregating the cellular distribution and fate of activated MLKL

The paper identifies LUBAC-mediated M1 ubiquitination as a species-specific checkpoint regulating necroptosis by controlling MLKL membrane accumulation in human cells and organoids.

Bioprinting the Tumor Microenvironment with an Upgraded Consumer Stereolithographic 3D Printer

This paper describes an upgraded consumer 3D printer for bioprinting tumor models with tunable mechanical properties and validated cell viability.

Long-term live imaging of epithelial organoids and corresponding multiscale analysis reveal high heterogeneity and identify core regulatory principles

The paper uses multiscale imaging and analysis of organoids to identify core regulatory principles of morphogenesis and reveal high heterogeneity in cell organization.

p63 sets the threshold for induction of apoptosis using a kinetically encoded ‘doorbell-like’ mechanism

The paper shows that TAp63 uses a slow third phosphorylation step to set a threshold for apoptosis induction in response to DNA damage.

Ultra-thin fluorocarbon foils optimize multiscale imaging of three-dimensional native and optically cleared specimens

This paper introduces ultra-thin fluorocarbon foils for improved multiscale imaging of 3D specimens, showing enhanced imaging quality and compatibility with various clearing media.