The paper identifies a new subgroup of light-driven sodium pumps with an additional glutamic acid residue that reduces pH sensitivity for sodium transport.

bioRxiv · Oct 11, 2023Biophysics

High Throughput Tomography (HiTT) on EMBL Beamline P14 on PETRA III

J. Albers, M. Nikolova, A. Svetlove, N. Darif, M. J. Lawson, T. R. Schneider, Y. Schwab, G. Bourenkov, E. Duke

The paper introduces HiTT, a high-throughput X-ray tomography system for rapid 3D imaging of biological samples, demonstrating its application on various tissues and its utility for population studies.

bioRxiv · Sep 6, 2023Bioengineering

Structural basis for receptor selectivity and inverse agonism in S1P5 receptors

E. Lyapina, E. Marin, A. Gusach, P. Orekhov, A. Gerasimov, A. Luginina, D. Vakhrameev, M. Ergasheva, M. Kovaleva, G. Khusainov, P. Khorn, M. Shevtsov, K. Kovalev, I. Okhrimenko, P. Popov, , V. Cherezov

The paper presents a high-resolution structure of the S1P5 receptor complexed with an inverse agonist, revealing structural features that explain receptor subtype selectivity and inverse agonism.

bioRxiv · Feb 25, 2022Biophysics

Serial femtosecond and serial synchrotron crystallography yield data of equivalent quality: a systematic comparison

P. Mehrabi, R. Buecker, G. Bourenkov, H. Ginn, D. von Stetten, H. M. Mueller-Werkmeister, A. Kuo, T. Morizumi, B. Eger, W.-L. Ou, S. Oghbaey, A. Sarracini, J. Besaw, O. Pare-Labrosse, S. Meier, , R. J. D. Miller

This study compares data quality from serial femtosecond and serial synchrotron crystallography for two proteins, finding equivalent quality regardless of radiation source.

bioRxiv · Aug 21, 2020Biophysics

Molecular mechanism of light-driven sodium pumping

K. Kovalev, R. Astashkin, I. Gushchin, P. Orekhov, D. Volkov, E. Zinovev, E. Marin, M. Rulev, A. Alekseev, A. Royant, P. Carpentier, S. Vaganova, C. Baeken, I. Sergeev, D. Zabelskii, , V. Gordeliy

The paper elucidates the molecular mechanism of Na+ pumping by KR2 rhodopsin using crystal structures and molecular dynamics, revealing a switching mechanism and structural similarities to channelrhodopsins.

bioRxiv · Jan 29, 2020Biophysics

High Resolution Structural Insights into Heliorhodopsin Family

K. Kovalev, D. Volkov, R. Astashkin, A. Alekseev, I. Gushchin, J. Haro-Moreno, A. Rogachev, T. Balandin, V. Borshchevskiy, A. Popov, G. Bourenkov, E. Bamberg, F. Rodriguez-Valera, G. Bueldt, V. Gordeliy

The paper presents high-resolution structures of two HeR-48C12 states, revealing unique features and suggesting potential enzymatic activity and involvement in redox processes.

bioRxiv · Sep 12, 2019Biophysics

A distributed lattice of aligned atoms exists in a protein structure: A hierarchical clustering study of displacement parameters in bovine trypsin

V. Ahlberg Gagner, I. Lundholm, M.-J. Garcia-Bonete, H. Rodilla, R. Friedman, V. Zhaunerchyk, G. Bourenkov, T. Schneider, J. Stake, G. Katona

This study examines how 0.5 THz irradiation affects atomic displacement parameters in bovine trypsin using hierarchical clustering to identify patterns in ADP similarities.

bioRxiv · Mar 13, 2019Biophysics