H. Y. Chang

This study shows that multiple extrachromosomal DNA species are coordinately inherited in cancer cells through mitotic co-segregation, enabling oncogene cooperation and guiding therapeutic strategies.

The paper identifies transcriptional correlates of ecDNA in tumors, showing upregulation of DNA repair and downregulation of immune pathways.

This study investigates chromosome loss in CRISPR-Cas9-engineered T cells and shows a modified manufacturing process reduces it while preserving editing efficacy.

The paper designs and engineers a high-yield, efficient recombinant peptide asparaginyl ligase (PAL) by modifying a consensus legumain sequence and applying the LAD hypothesis.

This study identifies the Mediator kinase module as a regulator of T cell differentiation and shows that its inhibition enhances effector T cell potency.

This study introduces CRISPR-CATCH, a method to purify and analyze extrachromosomal DNA, revealing hypomethylation and structural details of ecDNA in cancer cells.

The paper identifies ecDNA hubs as nuclear structures that drive cooperative oncogene expression through intermolecular enhancer-promoter interactions and shows BET inhibitors can disrupt these hubs and kill cancer cells.

This study shows that macrophage inflammatory and regenerative responses are phase-dependent on the cell cycle, with S-G2/M phases linked to tissue remodeling and reduced IFNG-induced repolarization.

This study examines chromatin accessibility in CD4+ T cells from SSc patients on MMF, finding sex and autoantibody-associated epigenetic differences linked to Th2 activation.

This study identifies CHD8 as a chromatin activator in human neurons that depends on the ERK-MAPK pathway effector ELK1 for its function.

This study identifies 309 host proteins interacting with SARS-CoV-2 RNA during infection and reveals their roles in virus-induced cell death and mitochondrial dysfunction.

This study shows that temporarily stopping CAR-T cell signaling can reverse exhaustion and improve their anti-tumor function through transcriptional and epigenetic changes.

This study shows that extrachromosomal DNA amplification of oncogenes is common in human cancers and is associated with worse patient outcomes.

APEX-seq maps subcellular RNA localization with nanometer resolution, revealing nuclear transcriptome organization and mitochondrial mRNA pathways.

This study tracks T cell clonal dynamics before and after PD-1 blockade in cancer patients, finding that new T cell clones, not pre-existing ones, expand in response to treatment.