J. S. Sharp

Exploring the Residue-Level Interactions between the R2ab Protein and Polystyrene Nanoparticles

This paper investigates residue-level interactions between the R2ab protein and polystyrene nanoparticles using mass spectrometry and HDX NMR, revealing conformational changes and supporting the adsorbotope model.

Structural Analysis of Phosphorylation Proteoforms in a Dynamic Heterogeneous System Using Flash Oxidation Coupled In-Line with Ion Exchange Chromatography

This study uses IEX LC-FOX to analyze structural changes in phosphoproteoforms of ovalbumin during dephosphorylation, revealing residue-level oxidation differences and structural stabilization effects.

Validated Determination of NRG1 Ig-like Domain Structure by Mass Spectrometry Coupled with Computational Modeling

The paper uses HR-HRPF-based modeling to determine the structure of NRG1 Ig-like domain, validating it against NMR results.

Differential response to prey quorum signals indicates predatory range of myxobacteria

This study compares how two predatory myxobacteria respond to different quorum signals from prey, revealing common and unique responses that suggest specialization in prey predation.

Inline Liquid Chromatography-Fast Photochemical Oxidation of Proteins Allows for Targeted Structural Analysis of Conformationally Heterogeneous Mixtures

The paper introduces LC-FPOP to analyze conformationally heterogeneous protein mixtures, showing it provides accurate conformer-specific footprints compared to pure samples.

Self-Organized Amphiphiles are Poor Hydroxyl Radical Scavengers in Fast Photochemical Oxidation of Proteins Experiments

This study investigates whether membrane environments or amphiphiles scavenge hydroxyl radicals in FPOP experiments, finding no significant bulk scavenging effect.

Effective Inhibition of SARS-CoV-2 Entry by Heparin and Enoxaparin Derivatives

This study evaluates the antiviral activity of heparin and enoxaparin derivatives against SARS-CoV-2 by testing their ability to neutralize pseudotyped virus particles.

Cryptic, solo acylhomoserine lactone synthase from predatory myxobacterium suggests beneficial contribution to prey quorum signaling

The paper identifies an orphaned AHL synthase from a predatory myxobacterium and shows it produces AHL signals under heterologous expression, suggesting a role in interspecies communication.

Rapid Quantification of Peptide Oxidation Isomers from Complex Mixtures

This paper introduces a new chromatography method to improve residue-level quantification of peptide oxidation isomers in HRPF data by co-eluting isomers and simplifying MS/MS analysis.

Intrinsic Buffer Hydroxyl Radical Dosimetry Using Tris(Hydroxymethyl)Aminomethane

The paper investigates Tris buffer's behavior as a hydroxyl radical dosimeter in FPOP experiments, finding it can enhance signal detection and enable real-time monitoring.

Salt-free fractionation of complex isomeric mixtures of glycosaminoglycan oligosaccharides compatible with ESI-MS and microarray analysis

This paper introduces a multi-dimensional separation method for glycosaminoglycan oligosaccharides that enables structural and functional analysis without desalting.

Real Time Normalization of Fast Photochemical Oxidation of Proteins Experiments by Inline Adenine Radical Dosimetry

This paper introduces real-time radical dosimetry for FPOP experiments to normalize hydroxyl radical concentration, enabling reproducible protein footprinting across varying buffer conditions.

Quantitative Protein Topography Measurements By High Resolution Hydroxyl Radical Protein Footprinting Enable Accurate Molecular Model Selection

This paper introduces HR-HRPF to measure solvent accessible surface area and evaluates its ability to distinguish accurate molecular models.