M. Pabst

Exploring the metabolic potential of Aeromonas to utilise the carbohydrate polymer chitin

This study shows two Aeromonas strains can degrade and use chitin as a carbon and nitrogen source, revealing their metabolic potential in nutrient-poor environments.

Full-Length Single-Molecule Protein Fingerprinting

This paper introduces a method to map amino acid positions and post-translational modifications in single protein molecules with sub-nanometer resolution using FRET-based single-molecule protein fingerprinting.

Aerobic denitrification as N2O source in microbial communities

The paper quantifies aerobic denitrification's contribution to N2O emissions in microbiomes with frequent oxic/anoxic transitions, showing it can respire a third of organic substrate with nitrate and produce up to a quarter of aerobically respired nitrate as N2O.

Meta-omics profiling of full-scale groundwater rapid sand filters explains stratification of iron, ammonium and manganese removals

The paper uses metaproteomics to analyze full-scale groundwater filters and shows how microbial protein expression stratifies with nutrient availability, explaining iron, ammonium, and manganese removal patterns.

Autoinsertion (LAiR): rapid functional reconstitution of integral membrane proteins into lipid bilayers

This paper introduces LAiR, a method for rapidly reconstituting integral membrane proteins into lipid bilayers, showing improved activity, stability, and preservation of protein properties compared to standard techniques.

Enrichment and application of bacterial sialic acids containing polymers from the extracellular polymeric substances of Candidatus Accumulibacter

This study identifies high molecular weight polysaccharides in Ca. Accumulibacter EPS as rich in NulOs and suggests their potential for sepsis treatment.

Turnover of the extracellular polymeric matrix in an EBPR microbial community

The paper measures EPS turnover in a PAO-enriched microbial community and finds similar turnover rates for EPS proteins and intracellular proteins, with no preferential consumption by other microbes.

Extracting and characterizing protein-free megabasepair DNA for in vitro experiments

The paper presents a method to isolate megabasepair-long deproteinated DNA for in vitro studies, enabling the investigation of chromosome organization using long DNA substrates.

Functional single cell proteomic profiling of cells with abnormal DNA damage response dynamics

The paper develops FUNpro, a functional single-cell proteomic profiling method, to link proteomes with dynamic cellular phenotypes, identifying PDS5A and PGAM5 in DDR survival.

Modular, synthetic chromosomes as new tools for large scale engineering of metabolism

This study demonstrates the use of modular neochromosomes in yeast to engineer metabolic pathways for anthocyanin production.

Adaptation of anammox bacteria to low temperature via gradual acclimation and cold shocks: distinctions in protein expression, membrane composition and activities

This study compares gradual temperature decrease and cold shocks for adapting anammox bacteria to low temperatures, finding cold shocks more effective in maintaining metabolic activity and protein expression.

Time grid-based isomer specific N-glycan analysis and detection of bisecting Lewis X in human brain

The paper introduces a method using stable isotope labeled reference N-glycans to resolve isomeric structures in complex mixtures, revealing new isomers in human brain N-glycans.

Investigating the chemolithoautotrophic and formate metabolism of Nitrospira moscoviensis by constraint-based metabolic modeling and 13C-tracer analysis

This study reconstructs a genome-scale metabolic model of Nitrospira moscoviensis and validates it using proteomics, 13C-tracer, and metabolomic analysis to elucidate its autotrophic and formate metabolism.

Production of nonulosonic acids in the extracellular polymeric substances of Candidatus Accumulibacter phosphatis

The paper investigates the production of nonulosonic acids (NulOs) in Candidatus Accumulibacter phosphatis biofilms using proteomic, mass spectrometry, and lectin-binding analyses, revealing their diversity and ecological significance.

Autotrophic and mixotrophic metabolism of an anammox bacterium revealed by in vivo 13C and 2H metabolic network mapping

This study uses isotope tracing and metabolomics to resolve the central carbon metabolism of an anammox bacterium, revealing discrepancies between genomic predictions and in vivo metabolic fluxes.