BiophysicsbioRxiv
Conformational dynamics of Cas9 governing DNA cleavage revealed by single molecule FRET
M. Yang, S. Peng, R. Sun…·C. Chen
This study uses single molecule FRET to show Cas9 undergoes conformational changes and long-range allosteric communication that regulate DNA cleavage, identifying key residues for improving CRISPR/Cas9 specificity.
DOI 10.1016/j.celrep.2017.12.048