N. J. Stonehouse

This study investigates how mutations at the 3B3-3C junction of FMDV affect polyprotein processing and replication, showing that altered processing impacts enzyme function and RNA interaction.

This study develops broad-spectrum anti-viral compounds targeting the conserved EV 2C protein, showing low IC50 values and suggesting a role for 2C in capsid assembly.

This study identifies an amino acid substitution in norovirus VP1 that enhances cellular attachment and alters tropism by affecting receptor recruitment and membrane mobility.

This study produces antigenically stable EVA71 virus-like particles in Pichia pastoris, showing they retain native antigenic conformation and elicit neutralising antibodies comparable to inactivated virus vaccines.

This paper investigates hepatitis E virus polyprotein processing and finds that it relies on host proteases rather than viral proteases for replication.

This study produces protease-independent poliovirus VLPs in Pichia pastoris, showing they maintain native antigenicity and thermostability, suggesting potential for improved vaccine development.

This paper produces and characterizes stabilized PV-3 virus-like particles using Pichia pastoris, showing improved thermostability and antigen ratio compared to wild-type PV-3.

This study identifies a conserved glutathione binding site in poliovirus VLPs that can be targeted by antivirals and used to stabilize vaccines.

VelcroVax is a vaccine platform that enhances antibody titres against a viral glycoprotein in mice by combining a modified VLP with a SUMO-tagged antigen.

The paper investigates differences in conformational constraints on negative strand RNA folding among positive strand RNA viruses, finding significant variation in ribozyme activity and duplex formation.

The paper determines the structure of P. pastoris fatty acid synthase using cryoEM, identifying structural differences from S. cerevisiae FAS and resolving the mobile ACP domain.

This study identifies higher-order structures of the foot-and-mouth disease virus RdRp and their role in RNA replication through cryo-EM and mutagenesis.

This study shows that the CHIKV nsP3 macro domain inhibits NF-κB activation by blocking TNF-mediated signaling, with effects dependent on ADP-ribosyl hydrolase activity.

This study uses cryo-EM to reveal the dynamic conformational changes in murine norovirus capsids, showing flexibility in protruding domains that impact virion stability and infectivity.