Author

A. Kotecha

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 6 Microbiology· 4 Biochemistry· 2

Signature Assays, Organisms & Methods

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Assay:cryo-EM· 11 Approach:experimental assay· 9 Approach:microscopy & imaging analysis· 6 Research Focus:protein structure prediction· 6 Research Focus:host-pathogen & immune response· 4 Organism:virus· 4 Research Focus:protein folding & dynamics· 3 Research Focus:cell signaling & trafficking· 2

Frequent Co-Authors

D. Karia· 2 shared D. M. Kern· 2 shared D. Sun· 2 shared L. Yu· 2 shared P. Zhang· 2 shared S. G. Brohawn· 2 shared X. Fu· 2 shared A. B. Kleinpeter· 1 shared

Activity by Year

2020-2023 · peak 5/yr

Co-Author Collaboration Network

10 top collaborators · 8 mutual co-author links

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2 shared papersBiophysics

D. Karia

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Author profile →

12 papers

Recent·Most cited

Virion morphology and on-virus spike protein structures of diverse SARS-CoV-2 variants

Z. Ke, T. P. Peacock, J. C. Brown, C. M. Sheppard, T. I. Croll, A. Kotecha, D. H. Goldhill, W. S. Barclay, J. A. Briggs

The paper analyzes SARS-CoV-2 variant virion morphology and spike protein structures using cryo-EM, finding no adaptive changes in virion shape but multiple amino acid changes affecting spike conformational dynamics.

bioRxiv · Dec 21, 2023Microbiology

Conformational diversity in class C GPCR positive allosteric modulation

G. Cannone, L. Berto, F. Malhaire, G. Ferguson, A. Fouillen, S. Balor, J. Font-Ingles, A. Llebaria, C. GOUDET, A. Kotecha, V. R. Kutti, G. Lebon

This study reports cryoEM structures of mGlu5 receptor with different PAMs, showing how PAM binding modulates receptor conformation and function.

bioRxiv · Nov 7, 2023Biochemistry

Structural and quantum chemical basis for OCP-mediated quenching of phycobilisomes

P. V. Sauer, L. Cupellini, M. Sutter, M. Bondanza, M. A. Dominguez Martin, H. Kirst, D. Bina, A. F. Koh, A. Kotecha, B. J. Greber, E. Nogales, T. Polivka, B. Mennucci, C. A. Kerfeld

The paper reveals the structure of the OCP-PBS complex and elucidates the quantum mechanism of energy transfer during non-photochemical quenching in cyanobacteria.

bioRxiv · Sep 30, 2023Biophysics

Deep reconstructing generative networks for visualizing dynamic biomolecules inside cells

R. Rangan, S. Khavnekar, A. Lerer, J. Johnston, R. Kelley, M. Obr, A. Kotecha, E. D. Zhong

The paper introduces cryoDRGN-ET, a deep generative model for reconstructing dynamic biomolecular structures from cryo-ET subtomograms, revealing ribosome states in yeast cells.

bioRxiv · Aug 18, 2023 · PreprintBiophysics

Disease-specific tau filaments assemble via polymorphic intermediates

S. Lövestam, D. Li, J. L. Wagstaff, A. Kotecha, D. Kimanius, S. H. McLaughlin, A. G. Murzin, S. M. V. Freund, M. Goedert, S. H. W. Scheres

This study uses cryo-EM to analyze intermediate structures in tau filament assembly, revealing shared and condition-dependent intermediates in Alzheimer's and CTE pathogenesis.

bioRxiv · Jul 24, 2023Biophysics

Structure of SARS-CoV-2 M protein in lipid nanodiscs

K. A. Dolan, M. Dutta, D. M. Kern, A. Kotecha, G. A. Voth, S. G. Brohawn

The paper presents the cryo-EM structure of SARS-CoV-2 M protein in lipid nanodiscs, revealing its homodimer structure and potential role in viral assembly.

bioRxiv · Jun 12, 2022Biophysics

Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs

D. M. Kern, B. Sorum, S. S. Mali, C. M. Hoel, S. Sridharan, J. P. Remis, D. B. Toso, A. Kotecha, D. M. Bautista, S. G. Brohawn

This study presents the cryo-EM structure of SARS-CoV-2 3a ion channel in lipid nanodiscs, revealing its functional properties and evolutionary conservation.

Nature Structural & Molecular Biology · Jun 22, 2021Peer-reviewedBiophysics

Evolving cryo-EM structural approaches for GPCR drug discovery

X. Zhang, R. M. Johnson, I. Drulyte, L. Yu, A. Kotecha, R. Danev, D. Wootten, P. M. Sexton, M. J. Belousoff

This study validates stable GPCR:Gs complexes without Nb35 stabilizers and evaluates 200kV vs 300kV cryo-EM imaging for drug discovery.

bioRxiv · Jan 11, 2021Biochemistry

CryoET structures of immature HIV Gag reveal a complete six-helix bundle and stabilizing small molecules distinct from IP6

L. Mendonca, D. Sun, J. Ning, J. Liu, A. Kotecha, M. Olek, T. Frosio, X. Fu, B. A. Himes, A. B. Kleinpeter, E. O. Freed, J. Zhou, C. Aiken, P. Zhang

The paper determines the structure of the HIV Gag six-helix bundle using cryoET and identifies small molecules stabilizing the immature lattice.

bioRxiv · Oct 31, 2020 · PreprintMicrobiology

Single-particle cryo-EM at atomic resolution

T. Nakane, A. Kotecha, A. Sente, G. McMullan, S. Masiulis, P. M. G. E. Brown, I. T. Grigoras, L. Malinauskaite, T. Malinauskas, J. Miehling, L. Yu, D. Karia, E. V. Pechnikova, E. de Jong, J. Keizer, , S. Scheres

This paper demonstrates achieving atomic resolution cryo-EM structures for membrane proteins and apo-ferritin using advanced hardware, enabling detailed structural analysis and hydrogen bonding visualization.

Nature · Oct 21, 2020Peer-reviewedBiophysics

Assembly intermediates of orthoreovirus captured in the cell

G. Sutton, D. Sun, X. Fu, A. Kotecha, C. W. Hecksel, D. K. Clare, p. zhang, D. I. Stuart, M. Boyce

The paper visualizes a novel single-shelled icosahedral intermediate of mammalian orthoreovirus using cryo-electron tomography, revealing structural insights into viral assembly.

bioRxiv · Jun 10, 2020Microbiology

A high resolution view of an adolescent flavivirus

M. Renner, W. Dejnirattisai, L. Carrique, I. Serna Martin, D. Karia, S. L. Ilca, S. F. Ho, A. Kotecha, J. R. Keown, J. Mongkolsapaya, G. R. Screaton, J. M. Grimes

This study presents high-resolution cryo-EM structures of immature and mature flaviviruses, revealing structural changes during maturation and the role of pH in conformational rearrangements.

bioRxiv · Jun 7, 2020 · PreprintMicrobiology