A. Cheng

Targeting NOTCH2-JAG1 juxtacrine signaling reverses macrophage-mediated tumor resistance to taxol

This study identifies NOTCH2-JAG1 juxtacrine signaling as a target to reverse macrophage-mediated taxane resistance in ovarian cancer by sensitizing tumors to paclitaxel.

Effects of intensified training with insufficient recovery on joint level and single muscle fibre mechanical function: The role of myofibrillar Ca2+ sensitivity

This study shows that intensified training with insufficient recovery reduces myofibrillar Ca2+ sensitivity in single muscle fibers, contributing to impaired neuromuscular function at lower activation frequencies.

Requirement of GrgA for Chlamydia infectious progeny production, optimal growth, and efficient plasmid maintenance

This paper shows that GrgA is essential for Chlamydia growth, plasmid maintenance, and differentiation, and identifies its role in regulating sigma factors and gene expression.

Human cytomegalovirus mediates APOBEC3B relocalization early during infection through a ribonucleotide reductase-independent mechanism

This paper shows that human cytomegalovirus (HCMV) relocalizes APOBEC3B from the nucleus to the cytoplasm through an RNR-independent mechanism, unlike related herpesviruses.

Ras-dependent RAF-MAPK hyperactivation by pathogenic RIT1 is a therapeutic target in Noonan syndrome-associated cardiac hypertrophy

This study identifies how pathogenic RIT1 mutations drive RAF-MAPK hyperactivation in Noonan syndrome, showing MEK inhibition can alleviate cardiac hypertrophy in a mouse model.

Lung Tumor Microphysiological System with 3D Endothelium to Evaluate Modulators of T-Cell Infiltration

This paper introduces a 3D lung tumor on chip model with endothelium to evaluate T-cell infiltration modulators and demonstrates its utility in assessing immune chemotaxis and vascular responses.

Strain dropouts reveal interactions that govern the metabolic output of the gut microbiome

The paper investigates how strain dropouts in the gut microbiome affect community metabolism and host physiology, finding that functionally redundant strains can have significant impacts outside their niche.

Fully Automated Multi-Grid Cryo-EM Screening using Smart Leginon

Smart Leginon automates cryo-EM grid screening, reducing operator time from 6 hours to <10 minutes and improving throughput.

ct2vl: Converting Ct Values to Viral Loads for SARS-CoV-2 RT-qPCR Test Results

The paper introduces ct2vl, a Python package that converts RT-qPCR Ct values to viral loads for SARS-CoV-2 tests, enabling quantitative analysis of test results.

Epsin2, a novel target for multiple system atrophy therapy via α-synuclein/FABP7 propagation

This study identifies epsin-2 as a therapeutic target for multiple system atrophy by regulating FABP7/-synuclein hetero-aggregates propagation.

Expression and functional evaluation of recombinant human ABO blood group antigen cleaving glycoside hydrolases α-N-acetylgalactosaminidase, α-galactosidase, and endo-β-galactosidase produced in Escherichia coli

This study evaluates recombinant glycoside hydrolases for converting A, B, and AB blood types to O by cleaving surface antigens, showing partial success in porcine red blood cells.

Mapping the T cell repertoire to a complex gut bacterial community

The paper maps T cell responses to a complex gut bacterial community and identifies a conserved antigen recognized by multiple TCRs.

Evidence linking APOBEC3B genesis and evolution of innate immune antagonism by gamma-herpesvirus ribonucleotide reductases

The paper investigates how gamma-herpesvirus ribonucleotide reductases antagonize APOBEC3B, showing conservation across human and Old World monkey-infecting viruses but not New World monkey-infecting viruses.

Identification of a promiscuous conserved CTL epitope within the SARS-CoV-2 spike protein

The paper identifies a conserved CTL epitope in the SARS-CoV-2 spike protein that is compatible with most HLA alleles and suitable for evaluating CD8+ T cell responses.

System Environmental Metrics Collector for EM facilities

The paper develops a system for monitoring environmental metrics in cryoEM facilities to optimize instrument uptime and data production.

CryoEM structure of the EBV ribonucleotide reductase BORF2 and mechanism of APOBEC3B inhibition

This study reveals how EBV's BORF2 protein inhibits APOBEC3B by blocking its access to DNA, providing insights for drug development.

Monosodium Urate Crystals regulate a unique JNK-dependent macrophage metabolic and inflammatory response

This study shows that monosodium urate crystals induce a unique JNK-dependent metabolic and inflammatory response in macrophages, distinct from LPS, and that JNK inhibition reduces inflammation in gout models.

The RAS GTPase RIT1 compromises mitotic fidelity through spindle assembly checkpoint suppression

This study shows that RIT1, a Ras GTPase, suppresses the spindle assembly checkpoint, leading to mitotic errors and chromosome segregation defects.

Effects of Controlled Dual Growth Factor Delivery on Bone Regeneration Following Composite Bone-Muscle Injury

This study evaluates a smart delivery system for dual growth factor (BMP-2 and VEGF) release in composite bone-muscle injuries, finding no synergistic effect but improved bone healing with low-dose delivery.

Arginine depletion through ADI-PEG20 to treat argininosuccinate synthase deficient ovarian cancer, including small cell carcinoma of the ovary, hypercalcemic type

This paper identifies ASS1 deficiency in rare ovarian cancers and shows that ADI-PEG20 inhibits tumor growth in ASS1-deficient models.

A dynamic control of human telomerase holoenzyme

The paper demonstrates that human telomerase holoenzymes have fast- and slow-acting catalytic sites that become inactive after two rounds of catalysis, and can be reactivated by intracellular factors.

High Resolution Single Particle Cryo-Electron Microscopy using Beam-Image Shift

This study evaluates the impact of beam-image shift on cryo-EM single-particle reconstruction quality by testing different beam tilt angles and analyzing FSC values and water density peaks.

Routine Determination of Ice Thickness for Cryo-EM Grids

The paper presents two methods to measure ice thickness in cryo-EM grids during data collection to improve sample preparation and data quality.

Gut bacterial metabolite Urolithin A (UA) mitigates Ca2+ 1 entry inT cells by regulating miR-10a-5p

This study shows that gut metabolite UA reduces T cell proliferation by increasing miR-10a-5p and decreasing Orai1/STIM1/STIM2 expression and SOCE in murine CD4+ T cells.

Routine Single Particle CryoEM Sample and Grid Characterization by Tomography

This study uses fiducial-less tomography to analyze cryoEM grids and finds that 90% of particles are adsorbed to air-water interfaces, impacting protein structure and single particle analysis.